[Histonet] Derm processing times

[mari.ann.mailhiot <@t> leica-microsystems.com]

Jennifer

Processing time for skin can very. It sounds like you are going to process
all sizes.

The most important issue is always fixation. In most cases that is never a
problem with skin.

I have a protocol for small pieces one and two milimeters. I also have on
for 3 milimeters and above. They have always worked the best for me.

I also have used xylene substitutes instead of xylene on my skin protocols.
I usually just cut the 95% alcohol down to two bottles Then I could have an
extra bottle for xylene subs. I always prefered 3 stations of a substitute,
but I do know some people only have two xylene subs.

I will attach to the bottom of my email.

Best Regards

(See attached file: skin Processing Protocol template.doc)

Mari Ann Mailhiot BA HT ASCP
Application Specialist/Trainer
Leica Microsystems
Biosystems Division
Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot <@t> leica-microsystems.com
www.leica-microsystems.com


                                                                           
             "jennifer cresor                                              
             mike hough"                                                   
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Hello all,

I am looking for some feed back on tissue processing times that have worked
well. The processor would be strictly dermatology specimens from small to
large. Your input is appreciated. Thank you,

Jennifer Cresor
jencres <@t> ca.rr.com

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