[Histonet] RE: Procedure for Immunoperoxidase on a Frozen Section

[Sorenson, Jon (Nampa)]

This is how we handle Frozen sections and touch preps for IHC:

Frozen tissue is picked up on "Plus" slides, the sections are air dried for

a approximately 1 hour and then fixed in room temperature acetone, and

finally air-dried again. These slides can now be used or stored in a

freezer for later use.

 

Fine Needle Aspirates (FNA's), Cytospins and Touch Preps

These specimens are prepared by Cytology and submitted for staining in

95% ethanol, they are rinsed in TBS and stained routinely.

 

Please Note: Fine Needle Aspirations, Cytospins, touch preps and frozen

sections need to have procedures that include protease treatment or antigen

retrieval modified so as not to include either, they are unnecessary and will be

detrimental to the tissue.

 

 
Jon Sorenson
Histology Coordinator
Mercy Medical Center
 
JonSorenson <@t> chiwest.com
208-463-5267
 

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