[Histonet] RE: Histonet Digest, Vol 62, Issue 36
Webb, Dorothy L
Dorothy.L.Webb <@t> HealthPartners.Com
Thu Jan 29 13:14:09 CST 2009
The sending of this issue only made it through #8..where did the rest go??
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, January 29, 2009 12:01 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 62, Issue 36
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Today's Topics:
1. Plastic embedding (Armando Tellez)
2. Need any info for JUNG HISTOSTAINER Ig (Michelle MacVeigh-Aloni)
3. MAD2 Immunocytochemistry (Yobou IG)
4. Re: Histonet Digest, Vol 62, Issue 35 (Marilyn.A.Weiss <@t> kp.org)
5. Recycled solvents and chatter (Dunn, Cyndy)
6. 2nd patient identifier on cassettes (Fye Beth)
7. RE: Plastic embedding (Molinari, Betsy)
8. RE: nuclear counterstain (Swain, Frances L)
9. Patient identifier (Webb, Dorothy L)
10. Klotskin's modification of the Masson's Trichrome (Pat Laurie)
11. Re: Klotskin's modification of the Masson's Trichrome
(koellingr <@t> comcast.net)
12. FW: [Histonet] Klotskin's modification of the Masson's
Trichrome (Lester Raff MD)
13. Re: FW: [Histonet] Klotskin's modification of the Masson's
Trichrome (Pat Laurie)
14. Re: FW: [Histonet] Klotskin's modification of the Masson's
Trichrome (Bryan Llewellyn)
15. PIN4 Control (thisisann <@t> aol.com)
16. Re: PIN4 Control (Rene J Buesa)
17. quiq decalcifier (Ben Spirto)
18. Georgia Society for Histotechnology Program (Shirley Powell)
----------------------------------------------------------------------
Message: 1
Date: Wed, 28 Jan 2009 17:30:47 -0500
From: "Armando Tellez" <atellez <@t> crf.org>
Subject: [Histonet] Plastic embedding
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <D48BEC1869EA534288B5F670DFA6E97301CA07B8 <@t> be018.mail.lan>
Content-Type: text/plain; charset="us-ascii"
Hi,
Does anyone have a protocol for plastic embedding that shows in detail
the procedure and equipment needed for this purpose that does not mind
share it? My office email is attached to my signature. Please let me
know since my boss want to start a stent pathology protocls and he
claims that we have the equipment, so first I need a good full blown
descriptive protocol and all the protocols in internet are already
assuming that you have some plastic embedding knowledge and I have never
embed any metal devices. (I need a protocol for microtome, not grinding)
Thanks
Armando Tellez
atellez <@t> crf.org <mailto:atellez <@t> crf.org>
Pathology Department
Tel: (845) 290 8034
Fax: (845) 290 8030
------------------------------
Message: 2
Date: Wed, 28 Jan 2009 17:38:13 -0800
From: Michelle MacVeigh-Aloni <macveigh <@t> usc.edu>
Subject: [Histonet] Need any info for JUNG HISTOSTAINER Ig
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <001301c981b2$40370840$5c237d80 <@t> DFS66DD1>
Content-Type: text/plain; charset=iso-8859-1
Hi all,
We do research. We got a hand me down JUNG AUTOSTAINER XL which now does all the H&E and it is a blessing! Now I am thinking about purchasing a second hand Immunostainer to run some immunos, so we can use our time for something better than babysitting the slides.
There is a very inexpensive JUNG HISTOSTAINER Ig available, but I have no experience in this area. (I couldn't even find a picture of it on the Internet...) For now we only do a TUNEL stain for apoptosis, but the need for more immunos is rising. (Our students use a lot of fluorescent markers if this makes any difference.)
What do you think? Do you recommend it or am I just investing in a pricey incubation chamber?
I would appreciate any input
Michelle
------------------------------
Message: 3
Date: Thu, 29 Jan 2009 03:27:17 -0800 (PST)
From: Yobou IG <digakuinsei <@t> yahoo.com>
Subject: [Histonet] MAD2 Immunocytochemistry
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <989360.3605.qm <@t> web58505.mail.re3.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Dear All
I am trying to detect kinetochore localization of MAD2 induced by a microtubule depolymerizing agent in SW480 human cancer cells. I have used 2 different protocols (-20 oC 100% methanol fixation for 20 minutes or 4% formaldehyde fixation for 20 minutes, both followed by permeabilization with either 0.3% triton/PBS or 100 micog/mL digitonin/PBS) but unfortunately I got negative results. I had success with microtubules immunocytochemical staining using methanol fixation method and I demonstrated the induction of prometaphase arrest by my treament, but it seems that MAD2 immunostaining is not as easy as microtubules. I would greatly appreciate if anyone has an experience in MAD2 immunocytochemical staining provides me with a help.
thanks a lot
Regards,
Yobou I.G., M.Sc.
PhD graduate student
kyoto prefectural university of medicine
Japan
------------------------------
Message: 4
Date: Thu, 29 Jan 2009 04:34:49 -0800
From: Marilyn.A.Weiss <@t> kp.org
Subject: [Histonet] Re: Histonet Digest, Vol 62, Issue 35
To: histonet <@t> lists.utsouthwestern.edu
Cc: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OF434C1244.1B380237-ON8825754D.0044B0DF-8825754D.00451BA4 <@t> kp.org>
Content-Type: text/plain; charset="US-ASCII"
Please take off my previous posting of looking for Pathology Assistant's
and post that Kaiser is looking for 2 Pathologist's Assistant's. Send
resume to Diane.I.Johnson <@t> kp.org or call 619-528-5386. I would appreciate
that. Was in a hurry and wanted to help out my boss, so sorry for the
confusion. I certainly know the difference.
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------------------------------
Message: 5
Date: Thu, 29 Jan 2009 07:56:16 -0500
From: "Dunn, Cyndy" <CDunn <@t> mtsinai.on.ca>
Subject: [Histonet] Recycled solvents and chatter
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <2009D9CA27C1B8418B551D309223B74902617CFD <@t> mshmail16>
Content-Type: text/plain; charset="us-ascii"
Does anyone experience chatter they think originates from using recycled
solvents in their tissue processors? We have tried many things to eliminate
the chatter. The latest was removing the three recycled xylenes from the
processor and replacing them with fresh Commercial xylene. After doing this
the Pathologist was very pleased with the sections, no chatter in eight
blocks, three levels each.
------------------------------
Message: 6
Date: Thu, 29 Jan 2009 07:47:20 -0600
From: Fye Beth <Beth.Fye <@t> HCAhealthcare.com>
Subject: [Histonet] 2nd patient identifier on cassettes
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<938F8EC5A524D34EB5796E23E52781D3039F603529 <@t> NADCWPMSGCMS05.hca.corpad.net>
Content-Type: text/plain; charset="us-ascii"
We average over 400 cassettes a day, and put the Accession # on the top of the cassette, the last name on one side and tissue type on the other side. We are currently still doing all this by hand, but are looking into getting slide and cassette labelers. We started doing this as a matter of safety and not by any regulatory standard 5-6 years ago. It is a procedure that has saved us many times over the years. It is very rare to have cassettes that are mislabeled by both the accession # and patients name.
Beth A. Fye, CT (ASCP)
Pathology Technical Manager
HCA Richmond Hospital Laboratories
office: (804)228-6564
pager: (804)759-6929
<mailto:Beth.Fye <@t> hcahealthcare.com>
------------------------------
Message: 7
Date: Thu, 29 Jan 2009 07:49:48 -0600
From: "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu>
Subject: RE: [Histonet] Plastic embedding
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C0B5840F14DB5B4ABF55D82A623D1E1B2BC60B <@t> thimailexch.THI2.COM>
Content-Type: text/plain; charset="us-ascii"
You and the rest of the world Armando. Information like this is costly
and hard to come by. Best of luck none the less.
Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
Houston, TX 77225
832-355-6524
832-355-6812 (fax)
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Armando
Tellez
Sent: Wednesday, January 28, 2009 4:31 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Plastic embedding
Hi,
Does anyone have a protocol for plastic embedding that shows in detail
the procedure and equipment needed for this purpose that does not mind
share it? My office email is attached to my signature. Please let me
know since my boss want to start a stent pathology protocls and he
claims that we have the equipment, so first I need a good full blown
descriptive protocol and all the protocols in internet are already
assuming that you have some plastic embedding knowledge and I have never
embed any metal devices. (I need a protocol for microtome, not grinding)
Thanks
Armando Tellez
atellez <@t> crf.org <mailto:atellez <@t> crf.org>
Pathology Department
Tel: (845) 290 8034
Fax: (845) 290 8030
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Thu, 29 Jan 2009 08:02:20 -0600
From: "Swain, Frances L" <SwainFrancesL <@t> uams.edu>
Subject: RE: [Histonet] nuclear counterstain
To: "rjbuesa <@t> yahoo.com" <rjbuesa <@t> yahoo.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "Melissa Mazan"
<melissa.mazan <@t> tufts.edu>
Message-ID:
<5B6165D78AC14544974A844787B47E380703CCEF <@t> MAIL5.ad.uams.edu>
Content-Type: text/plain; charset=iso-8859-1
Hi Melissa: When definition is compromised I use a 1% light green counterstain for 30 seconds. My boss thinks it is great.
Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic Surgery
Center for Orthopaedic Research
Barton Research Building 2R28
4301 West Markham Street
Little Rock AR 72205
(501) 686-8739 PHONE
(501) 686-8987 FAX
swainfrancesl <@t> uams.edu email
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, January 28, 2009 3:52 PM
To: histonet <@t> lists.utsouthwestern.edu; Melissa Mazan
Subject: Re: [Histonet] nuclear counterstain
If you obtain the detail you need with the DAB, eliminate the counter stain altogether because really you do not need it.
René J.
--- On Wed, 1/28/09, Melissa Mazan <melissa.mazan <@t> tufts.edu> wrote:
From: Melissa Mazan <melissa.mazan <@t> tufts.edu>
Subject: [Histonet] nuclear counterstain
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, January 28, 2009, 3:11 PM
Hi all,
I'm staining mouse lung tissues with Dako's Ki67 using Vector ABC Elite
and DAB to visualize - I get lovely sharp black brown nuclei where appropriate.
There is almost no background staining. However, no matter how lightly I
counterstain, I lose the definition between DAB and counterstain - I have tried
hematoxylin and Nuclear Red - any suggestions? Thanks - Melissa
histonet-request <@t> lists.utsouthwestern.edu wrote:
> Send Histonet mailing list submissions to
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> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. Wax. (Ian Montgomery)
> 2. Green counterstain Issues (Breeden, Sara)
> 3. Re: Green counterstain Issues (Rene J Buesa)
> 4. pathology assistants (Marilyn.A.Weiss <@t> kp.org)
> 5. RE: pathology assistants (Charles.Embrey)
> 6. second patient identifier on tissue cassette (VOGT, LORI)
> 7. Re: second patient identifier on tissue cassette (Piero Nelva)
> 8. RE: Wax. (Jim Reilly)
> 9. RE: pathology assistants (Marilyn.A.Weiss <@t> kp.org)
> 10. RE: second patient identifier on tissue cassette (Mike Pence)
> 11. RE: second patient identifier on tissue cassette (VOGT, LORI)
> 12. RE: pathology assistants (Kimberly Tuttle)
> 13. NSH 2009 Winter Lunch and Learn (Brenda Royce)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 27 Jan 2009 18:14:14 -0000
> From: "Ian Montgomery" <ian.montgomery <@t> bio.gla.ac.uk>
> Subject: [Histonet] Wax.
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <28401324DEB141E78BD380083E48CDEA <@t> IBLS.GLA.AC.UK>
> Content-Type: text/plain; charset="us-ascii"
>
> Paraffin wax is giving a few problems with some tissues. What
is
> the current favourite from UK suppliers?
>
> Ian.
>
> Dr. Ian Montgomery,
>
> Histotechnology,
>
> I.B.L.S. Support Unit,
>
> Thomson Building,
>
> University of Glasgow,
>
> Glasgow,
>
> G12 8QQ.
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 27 Jan 2009 12:26:49 -0700
> From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
> Subject: [Histonet] Green counterstain Issues
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
<4D14F0FC9316DD41972D5F03C070908B017E666E <@t> nmdamailsvr.nmda.ad.nmsu.edu>
>
> Content-Type: text/plain; charset="us-ascii"
>
> My (working) light green counterstain (Sheehan, Theory & Practice...,
pg
> 187) does its thing unevenly. Given, a lot of our tissue is poorly
> fixed to begin with or possibly autolytic, but I get more staining
> around the periphery and little or no staining in the body of the
> section. What am I missing here? Thanks!
>
>
> Sally Breeden, HT(ASCP)
>
> NM Dept. of Agriculture
>
> Veterinary Diagnostic Services
>
> PO Box 4700
>
> Albuquerque, NM 87106
>
> 505-841-2576
>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 27 Jan 2009 12:46:17 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Green counterstain Issues
> To: histonet <@t> lists.utsouthwestern.edu, "Breeden, Sara"
> <sbreeden <@t> nmda.nmsu.edu>
> Message-ID: <132392.68543.qm <@t> web65705.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> That your tissue was fixed outside, but not inside, meaning, poor
penetration most likely because of less than adequate time in the fixative.
Increase the fixing time and most likely your staining unevenness will
disappear.
> René J.
>
> --- On Tue, 1/27/09, Breeden, Sara <sbreeden <@t> nmda.nmsu.edu> wrote:
>
> From: Breeden, Sara <sbreeden <@t> nmda.nmsu.edu>
> Subject: [Histonet] Green counterstain Issues
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Tuesday, January 27, 2009, 2:26 PM
>
> My (working) light green counterstain (Sheehan, Theory & Practice...,
pg
> 187) does its thing unevenly. Given, a lot of our tissue is poorly
> fixed to begin with or possibly autolytic, but I get more staining
> around the periphery and little or no staining in the body of the
> section. What am I missing here? Thanks!
>
>
> Sally Breeden, HT(ASCP)
>
> NM Dept. of Agriculture
>
> Veterinary Diagnostic Services
>
> PO Box 4700
>
> Albuquerque, NM 87106
>
> 505-841-2576
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Tue, 27 Jan 2009 13:31:58 -0800
> From: Marilyn.A.Weiss <@t> kp.org
> Subject: [Histonet] pathology assistants
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <OF59301E7B.7ED2E79E-ON8825754B.0075E9BC-8825754B.007648EB <@t> kp.org>
> Content-Type: text/plain; charset="US-ASCII"
>
> Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a
salaried position. They are a Monday-Friday position from 8:30-5. Great
benefits.If you have any questions or would like to send your resume, please
contact Diane I Johnson at Kaiser Permanente. at Diane.I.Johnson <@t> kp.org or
619-528-5386.
>
> NOTICE TO RECIPIENT: If you are not the intended recipient of this
e-mail, you are prohibited from sharing, copying, or otherwise using or
disclosing its contents. If you have received this e-mail in error, please
notify the sender immediately by reply e-mail and permanently delete this e-mail
and any attachments without reading, forwarding or saving them. Thank you.
>
>
> ------------------------------
>
> Message: 5
> Date: Tue, 27 Jan 2009 16:44:45 -0600
> From: "Charles.Embrey" <Charles.Embrey <@t> carle.com>
> Subject: RE: [Histonet] pathology assistants
> To:
<Marilyn.A.Weiss <@t> kp.org>, <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <44780C571F28624DBB446DE55C4D733A1FE637 <@t> EXCHANGEBE1.carle.com>
> Content-Type: text/plain; charset="us-ascii"
>
> Are the looking for Pathology Assistants or Pathologists' Assistants?
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Marilyn.A.Weiss <@t> kp.org
> Sent: Tuesday, January 27, 2009 3:32 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] pathology assistants
>
> Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a
>
> salaried position. They are a Monday-Friday position from 8:30-5. Great
benefits.If you have any questions or would like to send your resume, please
contact Diane I Johnson at Kaiser Permanente. at Diane.I.Johnson <@t> kp.org or
619-528-5386.
>
> NOTICE TO RECIPIENT: If you are not the intended recipient of this
e-mail, you are prohibited from sharing, copying, or otherwise using or
disclosing its contents. If you have received this e-mail in error, please
notify the sender immediately by reply e-mail and permanently delete this e-mail
and any attachments without reading, forwarding or saving them. Thank you.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 6
> Date: Tue, 27 Jan 2009 19:54:27 -0500
> From: "VOGT, LORI" <LVOGT <@t> lewistownhospital.org>
> Subject: [Histonet] second patient identifier on tissue cassette
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <CDB81EE571C31A4781E569F960782B32257D14 <@t> MAIL.lewistown.lcl>
> Content-Type: text/plain; charset="iso-8859-1"
>
> How are you small hospitals dealing with a second patient identifier on
the tissue cassette if you do not have a cassette printer/labeler? Is there an
easier way then hand writing patient name on cassette?
>
>
*****************************************************************************************
> Lori A. Vogt
> Technical Section Head/ Histology
> Lewistown Hospital
> 400 Highland Ave.
> Lewistown, PA 17044
> Phone-717-242-7213
> Fax-717-242-7540
> Web Site: http://www.lewistownhospital.org
>
> The Mission of the Lewistown Healthcare Foundation (LHF) is to provide
personal, high quality, economical health care that meets the needs of our
communities.
>
*****************************************************************************************
> The information contained in the electronic message is privileged and
confidential information intended for the use of the addressee listed above. If
you are neither the intended recipient or the employee or agent responsible for
delivering this information to the intended recipient, you are hereby notified
that any disclosure, copying, distribution, or taking of any action in reliance
on the content of this telecopied information is strictly prohibited. If you
receive this e-mail in error, please immediately notify us by telephone to
arrange for return of the original document.
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Wed, 28 Jan 2009 14:02:06 +1100
> From: "Piero Nelva" <pieronelva01 <@t> bigpond.com>
> Subject: Re: [Histonet] second patient identifier on tissue cassette
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <8A53D65BAF9F451AAAE37A424C590E6F <@t> pentium4>
> Content-Type: text/plain; format=flowed; charset="iso-8859-1";
> reply-type=original
>
> Before we had our cassette labeller, we wrote the first three letters only
of the surname on the side. Pretty awkward, but we had no other option.
>
> Piero Nelva
> Anatomical PAthology
> Monash Medical Centre
> Australia
>
>
> ----- Original Message ----- From: "VOGT, LORI"
<LVOGT <@t> lewistownhospital.org>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Wednesday, January 28, 2009 11:54 AM
> Subject: [Histonet] second patient identifier on tissue cassette
>
>
> How are you small hospitals dealing with a second patient identifier on
the tissue cassette if you do not have a cassette printer/labeler? Is there an
easier way then hand writing patient name on cassette?
>
>
*****************************************************************************************
> Lori A. Vogt
> Technical Section Head/ Histology
> Lewistown Hospital
> 400 Highland Ave.
> Lewistown, PA 17044
> Phone-717-242-7213
> Fax-717-242-7540
> Web Site: http://www.lewistownhospital.org
>
> The Mission of the Lewistown Healthcare Foundation (LHF) is to provide
personal, high quality, economical health care that meets the needs of our
communities.
>
*****************************************************************************************
> The information contained in the electronic message is privileged and
confidential information intended for the use of the addressee listed above. If
you are neither the intended recipient or the employee or agent responsible for
delivering this information to the intended recipient, you are hereby notified
that any disclosure, copying, distribution, or taking of any action in reliance
on the content of this telecopied information is strictly prohibited. If you
receive this e-mail in error, please immediately notify us by telephone to
arrange for return of the original document.
>
>
> _______________________________________________
> Histonet mailing list
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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>
>
--------------------------------------------------------------------------------
>
>
>
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> ------------------------------
>
> Message: 8
> Date: Wed, 28 Jan 2009 09:49:06 -0000
> From: "Jim Reilly" <j.h.reilly <@t> clinmed.gla.ac.uk>
> Subject: RE: [Histonet] Wax.
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
<FCD2109433379347B248142CAE948EDF175607 <@t> exchange-be6.centre.ad.gla.ac.uk>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello Ian
>
> In the GBRC we are using Histoplast PE from Thermo Scientific.
>
> Jim
>
> James H Reilly Sir Graeme Davies Building
> GBRC
> 120 university Place
> Glasgow
> G12 8TA
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ian
> Montgomery
> Sent: 27 January 2009 18:14
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Wax.
>
>
> Paraffin wax is giving a few problems with some tissues.
> What is the current favourite from UK suppliers?
>
> Ian.
>
> Dr. Ian Montgomery,
>
> Histotechnology,
>
> I.B.L.S. Support Unit,
>
> Thomson Building,
>
> University of Glasgow,
>
> Glasgow,
>
> G12 8QQ.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 9
> Date: Wed, 28 Jan 2009 05:06:22 -0800
> From: Marilyn.A.Weiss <@t> kp.org
> Subject: RE: [Histonet] pathology assistants
> To: Charles.Embrey <@t> carle.com
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <OFE9B900D0.D93652DF-ON8825754C.0047F718-8825754C.0047FEB0 <@t> kp.org>
> Content-Type: text/plain; charset="US-ASCII"
>
> 2 assistants.
> NOTICE TO RECIPIENT: If you are not the intended recipient of this
e-mail, you are prohibited from sharing, copying, or otherwise using or
disclosing its contents. If you have received this e-mail in error, please
notify the sender immediately by reply e-mail and permanently delete this e-mail
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>
>
>
>
> "Charles.Embrey" <Charles.Embrey <@t> carle.com> 01/27/2009
02:44 PM
>
> To
> Marilyn A Weiss/CA/KAIPERM <@t> KAIPERM,
<histonet <@t> lists.utsouthwestern.edu>
> cc
>
> Subject
> RE: [Histonet] pathology assistants
>
>
>
>
>
>
> Are the looking for Pathology Assistants or Pathologists' Assistants?
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Marilyn.A.Weiss <@t> kp.org
> Sent: Tuesday, January 27, 2009 3:32 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] pathology assistants
>
> Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a
>
> salaried position. They are a Monday-Friday position from 8:30-5. Great
benefits.If you have any questions or would like to send your resume, please
contact Diane I Johnson at Kaiser Permanente. at Diane.I.Johnson <@t> kp.org or
619-528-5386.
>
> NOTICE TO RECIPIENT: If you are not the intended recipient of this
e-mail, you are prohibited from sharing, copying, or otherwise using or
disclosing its contents. If you have received this e-mail in error, please
notify the sender immediately by reply e-mail and permanently delete this e-mail
and any attachments without reading, forwarding or saving them. Thank you.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 10
> Date: Wed, 28 Jan 2009 08:20:31 -0600
> From: "Mike Pence" <mpence <@t> grhs.net>
> Subject: RE: [Histonet] second patient identifier on tissue cassette
> To: "VOGT, LORI" <LVOGT <@t> lewistownhospital.org>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <661949901A768E4F9CC16D8AF8F2838C017A3A63 <@t> IS-E2K3.grhs.net>
> Content-Type: text/plain; charset="us-ascii"
>
> Where in the CAP Standard check list for Anatomic Pathology does it
> state that a second patient identifier must be on the tissue cassette?
>
> I don't remember reading that.
>
> Mike
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of VOGT,
> LORI
> Sent: Tuesday, January 27, 2009 6:54 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] second patient identifier on tissue cassette
>
>
> How are you small hospitals dealing with a second patient identifier on
> the tissue cassette if you do not have a cassette printer/labeler? Is
> there an easier way then hand writing patient name on cassette?
>
> ************************************************************************
> *****************
> Lori A. Vogt
> Technical Section Head/ Histology
> Lewistown Hospital
> 400 Highland Ave.
> Lewistown, PA 17044
> Phone-717-242-7213
> Fax-717-242-7540
> Web Site: http://www.lewistownhospital.org
>
> The Mission of the Lewistown Healthcare Foundation (LHF) is to provide
> personal, high quality, economical health care that meets the needs of
> our communities.
> ************************************************************************
> *****************
> The information contained in the electronic message is privileged and
> confidential information intended for the use of the addressee listed
> above. If you are neither the intended recipient or the employee or
> agent responsible for delivering this information to the intended
> recipient, you are hereby notified that any disclosure, copying,
> distribution, or taking of any action in reliance on the content of this
> telecopied information is strictly prohibited. If you receive this
> e-mail in error, please immediately notify us by telephone to arrange
> for return of the original document.
>
>
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Wed, 28 Jan 2009 09:45:14 -0500
> From: "VOGT, LORI" <LVOGT <@t> lewistownhospital.org>
> Subject: RE: [Histonet] second patient identifier on tissue cassette
> To: "Mike Pence" <mpence <@t> grhs.net>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <CDB81EE571C31A4781E569F960782B32257D15 <@t> MAIL.lewistown.lcl>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I know it is not a Cap requirement yet. JACHO is requiring 2 patient
identifier on specimens and I am looking to the future trickle down effect.
Thanks Dawn D. Schneider, HT(ASCP)your advice and insight it really helps.
>
> -----Original Message-----
> From: Mike Pence [mailto:mpence <@t> grhs.net]
> Sent: Wednesday, January 28, 2009 9:21 AM
> To: VOGT, LORI; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] second patient identifier on tissue cassette
>
>
> Where in the CAP Standard check list for Anatomic Pathology does it
> state that a second patient identifier must be on the tissue cassette?
>
> I don't remember reading that.
>
> Mike
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of VOGT,
> LORI
> Sent: Tuesday, January 27, 2009 6:54 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] second patient identifier on tissue cassette
>
>
> How are you small hospitals dealing with a second patient identifier on
> the tissue cassette if you do not have a cassette printer/labeler? Is
> there an easier way then hand writing patient name on cassette?
>
> ************************************************************************
> *****************
> Lori A. Vogt
> Technical Section Head/ Histology
> Lewistown Hospital
> 400 Highland Ave.
> Lewistown, PA 17044
> Phone-717-242-7213
> Fax-717-242-7540
> Web Site: http://www.lewistownhospital.org
>
> The Mission of the Lewistown Healthcare Foundation (LHF) is to provide
> personal, high quality, economical health care that meets the needs of
> our communities.
> ************************************************************************
> *****************
> The information contained in the electronic message is privileged and
> confidential information intended for the use of the addressee listed
> above. If you are neither the intended recipient or the employee or
> agent responsible for delivering this information to the intended
> recipient, you are hereby notified that any disclosure, copying,
> distribution, or taking of any action in reliance on the content of this
> telecopied information is strictly prohibited. If you receive this
> e-mail in error, please immediately notify us by telephone to arrange
> for return of the original document.
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>
> _______________________________________________
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 12
> Date: Wed, 28 Jan 2009 10:27:31 -0500
> From: "Kimberly Tuttle" <ktuttle <@t> umm.edu>
> Subject: RE: [Histonet] pathology assistants
> To: <Charles.Embrey <@t> carle.com>, <Marilyn.A.Weiss <@t> kp.org>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <498033130200001A00028F75 <@t> GWIA2.umm.edu>
> Content-Type: text/plain; charset=US-ASCII
>
> LOL! You didnt answer the question and theres a Big Difference
>
>
>
>>>> <Marilyn.A.Weiss <@t> kp.org> 01/28/09 8:09 AM >>>
>>>>
> 2 assistants.
> NOTICE TO RECIPIENT: If you are not the intended recipient of this
e-mail, you are prohibited from sharing, copying, or otherwise using or
disclosing its contents. If you have received this e-mail in error, please
notify the sender immediately by reply e-mail and permanently delete this e-mail
and any attachments without reading, forwarding or saving them. Thank you.
>
>
>
>
> "Charles.Embrey" <Charles.Embrey <@t> carle.com> 01/27/2009
02:44 PM
>
> To
> Marilyn A Weiss/CA/KAIPERM <@t> KAIPERM,
<histonet <@t> lists.utsouthwestern.edu>
> cc
>
> Subject
> RE: [Histonet] pathology assistants
>
>
>
>
>
>
> Are the looking for Pathology Assistants or Pathologists' Assistants?
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Marilyn.A.Weiss <@t> kp.org
> Sent: Tuesday, January 27, 2009 3:32 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] pathology assistants
>
> Kaiser in San Diego is looking for 2 qualified Pathology Assistants in a
>
> salaried position. They are a Monday-Friday position from 8:30-5. Great
benefits.If you have any questions or would like to send your resume, please
contact Diane I Johnson at Kaiser Permanente. at Diane.I.Johnson <@t> kp.org or
619-528-5386.
>
> NOTICE TO RECIPIENT: If you are not the intended recipient of this
e-mail, you are prohibited from sharing, copying, or otherwise using or
disclosing its contents. If you have received this e-mail in error, please
notify the sender immediately by reply e-mail and permanently delete this e-mail
and any attachments without reading, forwarding or saving them. Thank you.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> This e-mail and any accompanying attachments may be privileged,
confidential, contain protected health information about an identified patient
or be otherwise protected from disclosure. State and federal law protect the
confidentiality of this information. If the reader of this message is not the
intended recipient; you are prohibited from using, disclosing, reproducing or
distributing this information; you should immediately notify the sender by
telephone or e-mail and delete this e-mail.
>
>
>
>
> ------------------------------
>
> Message: 13
> Date: Wed, 28 Jan 2009 11:43:53 -0500
> From: "Brenda Royce" <Brenda <@t> nsh.org>
> Subject: [Histonet] NSH 2009 Winter Lunch and Learn
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <A84B26F30CE5B34284BD32EEE8BDFDEA337D09 <@t> nsh-sbs01.nsh.local>
> Content-Type: text/plain; charset="us-ascii"
>
> Registration is now open for the first NSH Winter Lunch & Learn,
> presented in partnership with Leica Microsystems, taking place February
> 28, 2009 at Jacksonville Community College in Jacksonville, FL.
> Attendees have an opportunity to attend 3 sessions and earn 4.5 contact
> hours in this half day program.
> Schedule:
> 8:00am - 9:00am: Registration 9:00am - 10:30am: Small Specimen Management
- In a Large Volume World
> 10:30am - 12:00am: The Development of Antibodies - Concept to Cancer
> 12:00pm - 12:30pm: Lunch Provided by NSH 12:30pm - 2:00pm: The Paradox of
Change in Histotechnology
> All sessions are presented by top rated NSH presenters Skip Brown, Leica
> BioSystems L.L.C.-St. Louis and Dr. Mark Rees, Leica Microsystems.
> Its a great inexpensive way to expand your knowledge & earn those
needed
> contact hours.
> Visit our website to: www.nsh.org
>
> Register Online
>
<https://www.nshonline.org/eweb/DynamicPage.aspx?webcode=EventInfo&Reg_e
> vt_key=6c37f294-9716-4f7c-a65e-4b7b89435387&RegPath=EventRegFees>
or
> Download a PDF of the Registration Form
> <http://tools.isovera.com/organizations/org/Brochure.pdf>
> We hope to see you there!
>
>
>
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 62, Issue 34
> ****************************************
>
-- Melissa R. Mazan, DVM, Diplomate ACVIM
Associate Professor and Director of Equine Sports Medicine
Department of Clinical Sciences
Tufts Cummings School of Veterinary Medicine
200 Westborough Road
North Grafton,MA 01536
tel: 508-839-5395
fax: 508-839-7903
email: melissa.mazan <@t> tufts.edu
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.
------------------------------
Message: 9
Date: Thu, 29 Jan 2009 08:07:35 -0600
From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
Subject: [Histonet] Patient identifier
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<0E394B648E5284478A6CCB78E5AFDA2705635D04 <@t> hpes1.HealthPartners.int>
Content-Type: text/plain; charset="us-ascii"
According to the "rule" regarding 2 patient identifiers, it is our
understanding that the 2 identifiers must be on the container label and
in the computer system upon accessioning. When the computer assigns the
surgical case number, all info is linked to that listed surgical
accessioned number and your 2 or more identifiers are pulled up with the
case. That also keeps within "HIPPA" guidelines to have patient
information openly available when blocks or slides are sent out or left
out in view. Otherwise, barcoding is the ideal way to place, view, and
tie all information needed!!
Dorothy Webb, HT
Regions Histology Technical Supervisor
651-254-2962
________________________________________
This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.
If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us.
------------------------------
Message: 10
Date: Thu, 29 Jan 2009 06:21:07 -0800
From: Pat Laurie <foreightl <@t> gmail.com>
Subject: [Histonet] Klotskin's modification of the Masson's Trichrome
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<bdfbc2370901290621j7bd9fee5ge229aa413ba14c95 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi Everyone,
Has anyone heard of the Klotskin's modification of the Masson's Trichrome?
I am working with a liver pathologist who wants me to try to work it up. I
can't seem to find it anywhere. I have tried numerous books, and scoured
the internet. Someone asked about 3 years ago on histonet, but there were
no replies. If anyone could help me I would appreciate it.
Thanks,
--
Patrick Laurie (HT, ASCP)
Cellnetix Pathology and Laboratories
Seattle, Wa
------------------------------
Message: 11
Date: Thu, 29 Jan 2009 14:31:53 +0000
From: koellingr <@t> comcast.net
Subject: Re: [Histonet] Klotskin's modification of the Masson's
Trichrome
To: Pat Laurie <foreightl <@t> gmail.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<012920091431.24615.4981BDD90001504B0000602722070229339D09020704040A0105 <@t> comcast.net>
Isn't a Klatskin tumor (of the biliary tree) spelled with A and not O? There are multiple pictures and references to Klatskin's Trichrome. Try A spelling and not O.
Ray Koelling
Research Pathology
PhenoPath Labs
Seattle
-------------- Original message ----------------------
From: Pat Laurie <foreightl <@t> gmail.com>
> Hi Everyone,
>
> Has anyone heard of the Klotskin's modification of the Masson's Trichrome?
> I am working with a liver pathologist who wants me to try to work it up. I
> can't seem to find it anywhere. I have tried numerous books, and scoured
> the internet. Someone asked about 3 years ago on histonet, but there were
> no replies. If anyone could help me I would appreciate it.
>
> Thanks,
>
> --
> Patrick Laurie (HT, ASCP)
> Cellnetix Pathology and Laboratories
> Seattle, Wa
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Thu, 29 Jan 2009 08:32:30 -0600
From: "Lester Raff MD" <LRaff <@t> uropartners.com>
Subject: FW: [Histonet] Klotskin's modification of the Masson's
Trichrome
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FE0368FE5AA6684DB5990BBEED742C531EF211 <@t> UPHQMSX01.uropartners.local>
Content-Type: text/plain; charset="us-ascii"
Try searching Klatskin Trichrome. Lots of references on internet.
Klatskin was a famous liver pathologist.
Lester J. Raff, MD
Medical Director
UroPartners Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel 708.486.0076
Fax 708.486.0080
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pat
Laurie
Sent: Thursday, January 29, 2009 8:21 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Klotskin's modification of the Masson's Trichrome
Hi Everyone,
Has anyone heard of the Klotskin's modification of the Masson's
Trichrome?
I am working with a liver pathologist who wants me to try to work it up.
I
can't seem to find it anywhere. I have tried numerous books, and
scoured
the internet. Someone asked about 3 years ago on histonet, but there
were
no replies. If anyone could help me I would appreciate it.
Thanks,
--
Patrick Laurie (HT, ASCP)
Cellnetix Pathology and Laboratories
Seattle, Wa
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 13
Date: Thu, 29 Jan 2009 07:10:25 -0800
From: Pat Laurie <foreightl <@t> gmail.com>
Subject: Re: FW: [Histonet] Klotskin's modification of the Masson's
Trichrome
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<bdfbc2370901290710j5870e076v698ebd8cc00a0911 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
I apologize for the typo, I meant Klatskin, It is very early in the morning
here...There are many references, and yes, a couple of photos of the stain,
but no actual procedure...
On Thu, Jan 29, 2009 at 6:32 AM, Lester Raff MD <LRaff <@t> uropartners.com>wrote:
> Try searching Klatskin Trichrome. Lots of references on internet.
> Klatskin was a famous liver pathologist.
>
>
>
> Lester J. Raff, MD
>
> Medical Director
>
> UroPartners Laboratory
>
> 2225 Enterprise Dr. Suite 2511
>
> Westchester, Il 60154
>
> Tel 708.486.0076
>
> Fax 708.486.0080
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pat
> Laurie
> Sent: Thursday, January 29, 2009 8:21 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Klotskin's modification of the Masson's Trichrome
>
>
>
> Hi Everyone,
>
>
>
> Has anyone heard of the Klotskin's modification of the Masson's
> Trichrome?
>
> I am working with a liver pathologist who wants me to try to work it up.
> I
>
> can't seem to find it anywhere. I have tried numerous books, and
> scoured
>
> the internet. Someone asked about 3 years ago on histonet, but there
> were
>
> no replies. If anyone could help me I would appreciate it.
>
>
>
> Thanks,
>
>
>
> --
>
> Patrick Laurie (HT, ASCP)
>
> Cellnetix Pathology and Laboratories
>
> Seattle, Wa
>
> _______________________________________________
>
> Histonet mailing list
>
> Histonet <@t> lists.utsouthwestern.edu
>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Patrick Laurie (HT, ASCP)
Virginia Mason Medical Center
Seattle, Wa
------------------------------
Message: 14
Date: Thu, 29 Jan 2009 08:07:43 -0800
From: "Bryan Llewellyn" <llewllew <@t> shaw.ca>
Subject: Re: FW: [Histonet] Klotskin's modification of the Masson's
Trichrome
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>, "Pat Laurie"
<foreightl <@t> gmail.com>
Message-ID: <BBA0F96A71A8485DA5B1350424A63F2B <@t> Compaq>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Klatskin's Modification of Masson Trichrome Stain
1. Hydrate paraffin sections
2. Harris's hematoxylin, 10 minutes
3. 95% ethanol, rinse parent hepatic fat fraction provides an index that can
be
4. Picric alcohol (saturated solution of picric acid in 95% ethanol), 10
minutes
5. Wash, 10 minutes
6. Ponceau (1% ponceau in 1% glacial acetic acid), 5 minutes
7. Phosphomolybdic acid (1%, aqueous), 5 minutes
8. Aniline blue (saturated solution of aniline blue in 2.5% glacial acetic
acid), 2 minutes
9. Phosphomolybdic acid, 5 minutes
10. 1% acetic acid, 5 minutes
11. 70% alcohol, 2 minutes
12. Dehydrate and mount
http://www3.interscience.wiley.com/cgi-bin/fulltext/106594374/PDFSTART
Bryan Llewewllyn
----- Original Message -----
From: "Pat Laurie" <foreightl <@t> gmail.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, January 29, 2009 7:10 AM
Subject: Re: FW: [Histonet] Klotskin's modification of the Masson's
Trichrome
>I apologize for the typo, I meant Klatskin, It is very early in the morning
> here...There are many references, and yes, a couple of photos of the
> stain,
> but no actual procedure...
>
> On Thu, Jan 29, 2009 at 6:32 AM, Lester Raff MD
> <LRaff <@t> uropartners.com>wrote:
>
>> Try searching Klatskin Trichrome. Lots of references on internet.
>> Klatskin was a famous liver pathologist.
>>
>>
>>
>> Lester J. Raff, MD
>>
>> Medical Director
>>
>> UroPartners Laboratory
>>
>> 2225 Enterprise Dr. Suite 2511
>>
>> Westchester, Il 60154
>>
>> Tel 708.486.0076
>>
>> Fax 708.486.0080
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pat
>> Laurie
>> Sent: Thursday, January 29, 2009 8:21 AM
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] Klotskin's modification of the Masson's Trichrome
>>
>>
>>
>> Hi Everyone,
>>
>>
>>
>> Has anyone heard of the Klotskin's modification of the Masson's
>> Trichrome?
>>
>> I am working with a liver pathologist who wants me to try to work it up.
>> I
>>
>> can't seem to find it anywhere. I have tried numerous books, and
>> scoured
>>
>> the internet. Someone asked about 3 years ago on histonet, but there
>> were
>>
>> no replies. If anyone could help me I would appreciate it.
>>
>>
>>
>> Thanks,
>>
>>
>>
>> --
>>
>> Patrick Laurie (HT, ASCP)
>>
>> Cellnetix Pathology and Laboratories
>>
>> Seattle, Wa
>>
>> _______________________________________________
>>
>> Histonet mailing list
>>
>> Histonet <@t> lists.utsouthwestern.edu
>>
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>
> --
> Patrick Laurie (HT, ASCP)
> Virginia Mason Medical Center
> Seattle, Wa
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Thu, 29 Jan 2009 11:15:41 -0500
From: thisisann <@t> aol.com
Subject: [Histonet] PIN4 Control
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8CB5043F733D762-1004-801 <@t> FWM-M26.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
Can someone let me know where I can purchase a good PIN4 control....also, what tissue do you use for the negative when you use one control for multiple cases.
Thanks,
Ann
------------------------------
Message: 16
Date: Thu, 29 Jan 2009 09:03:57 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] PIN4 Control
To: histonet <@t> lists.utsouthwestern.edu, thisisann <@t> aol.com
Message-ID: <894423.77079.qm <@t> web65705.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Each case ought to have its own negative control. If several cases are treated by the same antibody you can have one single positive control for that Ab, but the same does not apply to negative controls.
rené J.
--- On Thu, 1/29/09, thisisann <@t> aol.com <thisisann <@t> aol.com> wrote:
From: thisisann <@t> aol.com <thisisann <@t> aol.com>
Subject: [Histonet] PIN4 Control
To: histonet <@t> lists.utsouthwestern.edu
Date: Thursday, January 29, 2009, 11:15 AM
Can someone let me know where I can purchase a good PIN4 control....also, what
tissue do you use for the negative when you use one control for multiple cases.
Thanks,
Ann
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 17
Date: Thu, 29 Jan 2009 11:20:00 -0600
From: Ben Spirto <brod033 <@t> gmail.com>
Subject: [Histonet] quiq decalcifier
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<287d127c0901290920o6160fda1y7e277aac7adb4a6f <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Could someone tell me the best way for quick decalcification of parafin
embeded brain tissue. I know about treatment with HCl. Anything else? With
something, perhaps that can be found in the biochemical lab??? thanks
Ben
------------------------------
Message: 18
Date: Thu, 29 Jan 2009 12:18:24 -0500
From: Shirley Powell <POWELL_SA <@t> Mercer.edu>
Subject: [Histonet] Georgia Society for Histotechnology Program
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <21B2510769024081812D5D3A12987F77 <@t> powellsa1>
Content-Type: text/plain; charset=us-ascii
The Georgia Society for Histotechnology invites you to the 2009 meeting to
be held at Sea Palms Resort at St. Simons Island, Georgia, March 20-22,
2009. The program is below and the registration form can be downloaded from
www.histosearch.com/gsh soon or I will email you a copy personally. The
complete hotel information is already on the GSH website. Please call for
reservations now by calling the Sea Palms Resort. Be sure to tell them you
are attending the GSH meeting. The phone number is 1-800-841-6268. Visit
the web site at www.seapalms.com. Special GSH Room Rates are $99 for two
double beds and $109 for two double beds or a King bed Suites are available
as well as Villas.
March 20, 2009 - Friday
1 to 5 p.m.: HT/HTL Review Session for Students
5 to 7 p.m.: Meeting Registration
7 to 9 p.m.: Vendor Reception in Vendor Area
March 21, 2009 - Saturday
7:00-8:00 a.m.: Meeting Registration
8:30 a.m. to 12: Workshop #1 - Today's Artifacts - Tomorrow's Facts
8:30 a.m. to 12: Workshop #2 - Expense Analysis and Reduction in the IHC Lab
(10:00 - 10:30 a.m.: Break in Vendor Area)
12:00 - 1:00-GSH AWARDS LUNCHEON
1:00 to 4:30 p.m.: Workshop #3 - Decalcified and Undecalcified Bone:
Histology Techniques
1:00 to 4:30 p.m.: Workshop #4 - Basic Troubleshooting for Histology
Laboratory Equipment
(2:30 - 3:00 p.m.: Break in Vendor area)
4:30 p.m.: GSH General Membership Meeting
(GSH Board Meeting to Follow)
March 22, 2009 - Sunday
7:00-8:00 a.m.: Meeting Registration
8:30 to 12 a.m.: Workshop #5 - Commitment in the Workplace - What Does it
Mean to the Employee and the Employer
8:30 to 12 a.m.: Workshop #6 - Contemporary Trends in Immunohistochemistry
(10:00 - 10:30 a.m.: Break in Vendor Area)
WORKSHOP DESCRIPTIONS:
#1: Today's Artifacts - Tomorrow's Facts?
Lamar Jones, BS, HT(ASCP) - This workshop will teach the participant to
recognize and identify artifacts from the gross board, fixation, processing,
embedding, microtomy, staining, coverslipping and other areas of
histotechnology.
#2: Expense Analysis and Reduction in the IHC Lab
Joe Myers, - This workshop will discuss ways to cut expenses in the
immunohistochemistry laboratory without sacrificing quality.
#3: Decalcified and Undecalcified Bone: Histology Techniques
Vickie Kalscheur - The speaker will give an overview of Decalcified and
Undecalcified bone sample preparation for research histology. Specimen
collection, fixation, decalcification, processing, and more will be covered
using a wide variety of bone samples. The undecalcified component, again
discusses handling, preparation, and staining of undecalcified Plastic
embedded bone samples. Special and immunohistochemical staining of bone
specimens will be discussed. This talk will be casual and informative for
those working in clinical or research settings. Handouts will be provided.
#4: Basic Troubleshooting for Histology Laboratory Equipment
Jason Velasquez, Technical Engineer -This course will provide a basic
preventive maintenance guide that will assist users of histology equipment
in the upkeep and troubleshooting of their instruments. The type of
cleaning solvents that can and cannot be used will be discussed (along with
some pictures that show what happens when the wrong cleaning supplies are
used) and how and where to clean for best results. The types of tools that
should be kept in the laboratory's tool chest and how and when to use them
will be demonstrated. Common types of faults that can be reasonably
repaired by the average Histotech will be discussed and the ways, tools and
thoughts behind the troubleshooting process will be investigated. Some
symptoms that precede failures will be made known so that the users can
notify their bio-medical technicians or repair group of a pending failure,
before the instrument breaks completely.
#5: Commitment in the Workplace - What Does it Mean to the Employee and
Employer
Wanda Grace Jones, - Hospitals, Research Labs, and Private Laboratories
still struggle with continuous loss of employees and finding new employees
to fill these positions. Past research has isolated two variables that
impact employee turnover. 1st variable is employee's identification with
and involvement in an organization (how involved are you). 2nd variable is
the employee's perception of level of commitment an organization has to the
employee. We will discuss the attitude toward an organization which
attaches the person to an organization, the process by which the goals of
the organization and employee become integrated, building better
communication between employee and employer and cost associated when an
employee decides to leave.
#6: Contemporary Trends in Immunohistochemistry
Mary Cheles, MPH, HTL, DLM(ASCP) - The analysis of a patient has
historically relied on morphology and the evaluation of individual
antibodies on pathological tissue. Immunohistochemistry has been in
practice for the past 40 years. During that time, we have seen an evolution
from individual reagents to optimized systems and from manual staining
practices to fully automated options. Pathology and laboratory medicine is
changing faster than ever. In the future, personalized medicine will define
the effect of a therapy based on an individual's gene and protein profile.
What does this mean and where does the histology community fit in? This
workshop will briefly review immunohistochemistry basics, opportunities for
automation, process standardization, antibody validation, regulatory product
labeling and current proficiency testing.
Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director Histology
Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA 31207
Ph: 478-301-2374
Fx: 478-301-5489
------------------------------
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End of Histonet Digest, Vol 62, Issue 36
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