[Histonet] re: plastic embedding

[Kevin Egan]

I am in a similar situation as Armando. My PI recently told me to figure out how to do plastic embedding to view calcein/Xylenol orange staining. I've been following a procedure described in " Immunohistochemistry of matrix markers in Technovit 9100 New-embedded undecalcified bone sections" - Eur Cell Mater. 2003 Dec 31;6:57-71; discussion 71 . But I have yet to actually begin infiltrating the bones. If anyone can offer any knowledge and advice I would greatly appreciate it. 

Thanks, 
Kevin 

Kevin Egan 
Research Specialist 
Department of Medicine 
Division of Geriatric Medicine 
University of Pennsylvania School of Medicine 
Biomedical Research Building (BRB) II/III, Room 745 
421 Curie Boulevard 
Philadelphia, PA 19104-6160 
Telephone: 215-573-4005 

Message: 1 
Date: Wed, 28 Jan 2009 17:30:47 -0500 
From: "Armando Tellez" <atellez <@t> crf.org> 
Subject: [Histonet] Plastic embedding 
To: <histonet <@t> lists.utsouthwestern.edu> 
Message-ID: <D48BEC1869EA534288B5F670DFA6E97301CA07B8 <@t> be018.mail.lan> 
Content-Type: text/plain; charset="us-ascii" 

Hi, 



Does anyone have a protocol for plastic embedding that shows in detail 
the procedure and equipment needed for this purpose that does not mind 
share it? My office email is attached to my signature. Please let me 
know since my boss want to start a stent pathology protocls and he 
claims that we have the equipment, so first I need a good full blown 
descriptive protocol and all the protocols in internet are already 
assuming that you have some plastic embedding knowledge and I have never 
embed any metal devices. (I need a protocol for microtome, not grinding) 



Thanks 



Armando Tellez 

atellez <@t> crf.org <mailto:atellez <@t> crf.org> 

Pathology Department 

Tel: (845) 290 8034 

Fax: (845) 290 8030