FW: [Histonet] Klotskin's modification of the Masson's Trichrome

[Bryan Llewellyn]

Klatskin's Modification of Masson Trichrome Stain

1. Hydrate paraffin sections
2. Harris's hematoxylin, 10 minutes
3. 95% ethanol, rinse parent hepatic fat fraction provides an index that can 
be
4. Picric alcohol (saturated solution of picric acid in 95% ethanol), 10 
minutes
5. Wash, 10 minutes
6. Ponceau (1% ponceau in 1% glacial acetic acid), 5 minutes
7. Phosphomolybdic acid (1%, aqueous), 5 minutes
8. Aniline blue (saturated solution of aniline blue in 2.5% glacial acetic 
acid), 2 minutes
9. Phosphomolybdic acid, 5 minutes
10. 1% acetic acid, 5 minutes
11. 70% alcohol, 2 minutes
12. Dehydrate and mount

http://www3.interscience.wiley.com/cgi-bin/fulltext/106594374/PDFSTART

Bryan Llewewllyn


----- Original Message ----- 
From: "Pat Laurie" <foreightl <@t> gmail.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, January 29, 2009 7:10 AM
Subject: Re: FW: [Histonet] Klotskin's modification of the Masson's 
Trichrome


>I apologize for the typo, I meant Klatskin, It is very early in the morning
> here...There are many references, and yes, a couple of photos of the 
> stain,
> but no actual procedure...
>
> On Thu, Jan 29, 2009 at 6:32 AM, Lester Raff MD 
> <LRaff <@t> uropartners.com>wrote:
>
>> Try searching Klatskin Trichrome.  Lots of references on internet.
>> Klatskin was a famous liver pathologist.
>>
>>
>>
>> Lester J. Raff, MD
>>
>> Medical Director
>>
>> UroPartners Laboratory
>>
>> 2225 Enterprise Dr. Suite 2511
>>
>> Westchester, Il 60154
>>
>> Tel 708.486.0076
>>
>> Fax 708.486.0080
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pat
>> Laurie
>> Sent: Thursday, January 29, 2009 8:21 AM
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] Klotskin's modification of the Masson's Trichrome
>>
>>
>>
>> Hi Everyone,
>>
>>
>>
>> Has anyone heard of the Klotskin's modification of the Masson's
>> Trichrome?
>>
>> I am working with a liver pathologist who wants me to try to work it up.
>> I
>>
>> can't seem to find it anywhere.  I have tried numerous books, and
>> scoured
>>
>> the internet.  Someone asked about 3 years ago on histonet, but there
>> were
>>
>> no replies.  If anyone could help me I would appreciate it.
>>
>>
>>
>> Thanks,
>>
>>
>>
>> --
>>
>> Patrick Laurie (HT, ASCP)
>>
>> Cellnetix Pathology and Laboratories
>>
>> Seattle, Wa
>>
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>>
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>>
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>
>
>
> -- 
> Patrick Laurie (HT, ASCP)
> Virginia Mason Medical Center
> Seattle, Wa
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