[Histonet] Re: staining for manganese

[Benoît Delatour]

Hi,
There is a Timm stain - like protocol from Angenstein 
(Manganese-enhanced MRI reveals structural and functional changes in the 
cortex of Bassoon mutant mice. Angenstein F, Niessen HG, Goldschmidt J, 
Lison H, Altrock WD, Gundelfinger ED, Scheich H. Cereb Cortex. 2007 
Jan;17(1):28-36) using Na2S that shows some interesting results with 
manganese autometallography. The problem, as mentionned by RJ Buesa, 
concerns the specificity of the staining. Using this method in the past 
I observed staining increase in the brain following MnCl injection.

Regards,

Benoît Delatour

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>
> Message: 1
> Date: Mon, 26 Jan 2009 18:58:34 +0900
> From: "Gerard Spoelstra" <G.Spoelstra <@t> murdoch.edu.au>
> Subject: [Histonet] staining for manganese
> To: <histonet <@t> lists.utsouthwestern.edu>
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> Hi everybody,
>
> I have some fish specimens which have been exposed to toxic levels of manganese. The researcher is hoping to see manganese localised on the gill. Lillie mentions the use of benzidine to stain for manganese. I will try DAB, but my searching on the net has drawn a blank.
>
> Gerard Spoelstra
> veterinary histology
> Murdoch University
> Western Australia 
>