[Histonet] Autoflourescence

[Andrea Hooper]

10-15 um seems extremely thick for paraffin sections.
Can you get thinner sections? Say no thicker than 6um?
Unfortunately autofluorescence is a fact of life with FFPE sections, hence why people normally do not choose to do immunofluorescence on FFPE sections. Are you tethered to using immunofluorescence? There are alternatives.



----- Original Message -----
From: "Patten, Nicole (NIH/NIAAA) [F]" <pattennj <@t> mail.nih.gov>
Date: Thursday, January 15, 2009 4:36 pm
Subject: [Histonet] Autoflourescence

> Hi-
> 
> I am having a horrible time with autofluorescence in my human 
> brain FFPE
> tissue. I have been using Sudan Black which helps a little, but the
> background is still pretty bad. The tissue is usually cut at 10-15um.
> 
> Does anyone have any other suggestions? Has anyone tried 
> photobleachingthe tissue prior to staining with any success? I 
> attempted this once
> with no luck...
> 
> Any help would be very much appreciated! Thanks in advance. 
> 
> Nicole
> 
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