[Histonet] Autoflourescence

[Patten, Nicole (NIH/NIAAA) [F]]

Hi-

I am having a horrible time with autofluorescence in my human brain FFPE
tissue. I have been using Sudan Black which helps a little, but the
background is still pretty bad. The tissue is usually cut at 10-15um.

Does anyone have any other suggestions? Has anyone tried photobleaching
the tissue prior to staining with any success? I attempted this once
with no luck...

Any help would be very much appreciated! Thanks in advance. 

Nicole