[Histonet] prion contaminated tissue processing

Jan Shivers shive003 <@t> umn.edu
Thu Jan 15 10:48:07 CST 2009


Joe,

I do IHC for prion diseases in animals.  No, tissue processing will NOT 
render prion-infected tissue safe for sectioning under normal conditions. 
Incubating in formic acid won't even totally inactivate the abnormal 
protein.  We have to use a dedicated microtome in a dedicated room, wear 
nitrile gloves and disposable lab coats and booties, and stain the slides on 
dedicated autostainers.

As others have said, check out the CDC website for more information.

Jan Shivers
UMN Vet Diag Lab


----- Original Message ----- 
From: "Joe Hardin" <hardin <@t> oncology.wisc.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, January 15, 2009 9:29 AM
Subject: [Histonet] prion contaminated tissue processing


> Hi All,
> Does anyone know if tissue processing for paraffin embedding will render
> prion infected tissue safe for sectioning?
>


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