[Histonet] blocking aldehydes for staining

[Bryan Llewellyn]

Use an aldehyde block on the slides 
(http://stainsfile.info/StainsFile/stain/schiff/reaction-block.htm) as 
glutaraldehyde often has an active aldehyde group ready to react, then try a 
PAS using a Schiff's reagent made with acriflavine, proflavine or acridine 
orange (http://stainsfile.info/StainsFile/stain/schiff/pafs.htm and 
http://stainsfile.info/StainsFile/stain/schiff/pas-standard.htm).  After  10 
minutes in the Schiff's reagent after step 7, put the section in 1% acid 
alcohol for 5 minutes or so, then wash as in step 8 and carry on.

Bryan Llewellyn




----- Original Message ----- 
From: "Bill McManus" <bill.mcmanus <@t> usu.edu>
To: "Paula Pierce" <contact <@t> excaliburpathology.com>; 
<histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, January 14, 2009 12:14 PM
Subject: Re: [Histonet] blocking aldehydes for staining


> Paula:
>
> Do you know of any stains that fluoresce and stain starches?
>
> On Jan 14, 2009, at 1:11 PM, Paula Pierce wrote:
>
>> Congo Red is for amyloid.
>>
>>  I suggest you use an iodine solution to stain starch. It will stain  it 
>> black.
>>
>> From: Bill McManus <bill.mcmanus <@t> usu.edu>
>> To: histonet <@t> lists.utsouthwestern.edu
>> Sent: Wednesday, January 14, 2009 1:51:46 PM
>> Subject: [Histonet] blocking aldehydes for staining
>>
>>
>> >
>> >
>> > We are trying to use Congo Red to stain starch for confocal, but it
>> > is  staining everything.  The sample  is condensed milk and very
>> soft
>> > without fixation.  Does anyone know of a good method to block the
>> > glutaraldehyde so that the Congo Red will only stain the starch?
>>
>>
>> >
>> > Bill McManus
>> > Western Dairy Center
>> > Utah State University
>> > bill.mcmanus <@t> usu.edu
>> >
>>
>>
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>
> Bill McManus
> Western Dairy Center
> Utah State University
> bill.mcmanus <@t> usu.edu
>
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