[Histonet] Complement C3 for mouse FFPE

[Pritchard, Michele]

Kim:

When you say 'complement C3' do you mean cleavage products of C3 after C3 activation (C3b-iC3b/C3c) or C3 iteself?  C3b-iC3b/C3c are covalently attached to the surface of cells/pathogens, so I suspect this is what you would like to localize in your mouse tissue.  We use an antibody in frozen sections that works beautifully (Cell Sciences clone 2/11).  We have only limited experience with this clone in FFPE tissues.  It likely requires antigen retrieval; thus far, we have tried using proteinase K and that was not successful.  Because the frozen sections look so nice, we have not tried to localize C3b-iC3b/C3c in FFPE lately.  If I had it to try again, I would start with citrate buffer antigen retrieval in my microwave pressure cooker. 

Hope this helps.

---mtp  

Michele T. Pritchard, Ph.D.
Research Associate
Nagy Laboratory
Department of Pathobiology/NE40
Lerner Research Institute
Cleveland Clinic
9500 Euclid Avenue
Cleveland, OH 44195
 
phone:  216.444.8613
fax:  216.636.1493
 
email:  pritchm <@t> ccf.org
 
Lab location:
Lerner Research Institute
NE4-214

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Merriam
Sent: Wednesday, January 14, 2009 1:23 PM
To: Histonet
Subject: [Histonet] Complement C3 for mouse FFPE


Hi,
 
Can anyone recommend a good antibody for complement C3 on mouse FFPE tissue?
 
Thanks,
Kim

Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA


      
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