[Histonet] RE: Histogel embedding mouse brain for cryotomy or vibratome

[gayle callis]

Bob, 

So which type of sectioning are you doing now? Cryotomy or vibratome? And
how thick do you want the sections to be?  If frozen sections on snap frozen
mouse brain, simply turning the temperature up to -16C or so will make
sectioning much easier and get rid of the shredding, friable sections.
Also, are you fixing then sucrose cryoprotecting after NBF or 4%
paraformaldehyde fixation of the brain (can be either perfused or immersion
fixation)? 

Gayle Callis
HTL(ASCP)HT,MT
Bozeman MT