[Histonet] RE: Histonet Digest, Vol 62, Issue 15
Martin, Jessica
jmartin <@t> lrgh.org
Tue Jan 13 12:45:15 CST 2009
Good Morning:
?
Our lab is looking into getting a new processor at some point!? We are looking at the Excelsior ES Tissue Processor from Thermo Scientific. We were wondering if any of you Histol labs out there are using this machine and if so how you like it? Have you had any problems with it, ie. mechanical?? Is it saving you money? and also is anyone using the machine with the xylene free option?
?
Thanks so much! Have a great day!
?
Jessica Piche-Grocki, HT(ASCP)
Waterbury Hospital
We have been using the Excelsior for about two years now. It has been great. I would recommend it highly. We have not tried the xylene free processing yet. There have been a few mechanical problems here and there but I feel that is to be expected of any piece of equipment. This processor has saved us a ton of money as the reagents don't have to be changed as often. Try a demo of one I don't think you will be disappointed.
Jessica Martin HT, ASCP
(603) 524-3211 x-3231
jmartin <@t> lrgh.org
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu [histonet-request <@t> lists.utsouthwestern.edu]
Sent: Tuesday, January 13, 2009 1:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 62, Issue 15
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Today's Topics:
1. Undecalcified bone in MMA and antigen retrieval (Neil Hand?)
(Benjamin PERRON)
2. Technovit 9100 and partial vacuum (JP Rey)
3. Processing 3-dimensional cell cultures into paraffin
(Kathleen Roberts)
4. Re: Automatic Stainer/coverslipper (Sherwood, Margaret )
5. RE: bluing in tap water? (Smith Wanda)
6. RE: bluing in tap water? (Tony Henwood)
7. Rat and Mouse Bone histology methods and equipment--HELP
(Jamie E Erickson)
8. lab closing, equipment for sale (Cathy Mayton)
9. Commercial Haematoxylin (Tanya Fulton)
10. RE: Commercial Haematoxylin (Swain, Frances L)
11. RE: Undecalcified bone in MMA and antigen retrieval (Neil
Hand?) (Jack Ratliff)
12. RE: Technovit 9100 and partial vacuum (Jack Ratliff)
13. Job opportunity NJ (rmweber113 <@t> comcast.net)
14. Mechanism of Staining - Special Stains (SARAH REEVES)
15. Re: Commercial Haematoxylin (Rene J Buesa)
16. RE: Commercial Haematoxylin (Weems, Joyce)
17. Excelsior ES Tissue Processor (Jessica Piche)
18. X-Gal staining (Hamilton, Melissa)
19. RE: Commercial Haematoxylin (Cheryl)
20. Re: X-Gal staining (anh2006 <@t> med.cornell.edu)
21. Re: bluing in tap water? (anh2006 <@t> med.cornell.edu)
22. Spit and Diastase (Parker, Helayne)
23. RE: Processing 3-dimensional cell cultures into paraffin
(Monfils, Paul)
24. RE: Processing 3-dimensional cell cultures into paraffin
(Bartlett, Jeanine (CDC/CCID/NCZVED))
25. RE: Spit and Diastase (Monfils, Paul)
----------------------------------------------------------------------
Message: 1
Date: Mon, 12 Jan 2009 19:02:05 +0100 (CET)
From: Benjamin PERRON <perron.b <@t> wanadoo.fr>
Subject: [Histonet] Undecalcified bone in MMA and antigen retrieval
(Neil Hand?)
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <14389681.292778.1231783325413.JavaMail.www <@t> wwinf1a34>
Content-Type: text/plain; charset=UTF-8
Hi all,
I'm encountering problems while trying to do antigen retrieval (AR) on undecalcified bone (rat jaw) sections embedded in methylmetracrylate (MMA). Sections fall off the slides while heated.
Section are 4??m thick, deposit on APES or gelatine coated slides, and heating is perform after total deplastification and rehydratation (I tried microwaves and warming bath in citrate buffer pH=6.0).
Could some around here share theyre experience with AR on undecalcified bone sections and in particular tell me :
-does section thickness as an impact on sections detachment while heating (I know some are performing thinner section, but 3-4??m is the thinner we are (in routine) able to do with rat jaw)
-is theyre a particular coating for slides that would better fit that what we are using (APES or gelatine). Someone suggest me Poly-l-lysine.
All tips and advices from experimented in this field would be very helpfull.
Benjamin Perron
Student (Master in Cell Biology)
EA2496
Montrouge
France
------------------------------
Message: 2
Date: Mon, 12 Jan 2009 12:20:05 -0600
From: "JP Rey" <jp1000r <@t> hotmail.com>
Subject: [Histonet] Technovit 9100 and partial vacuum
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <COL0-DAV46627482F6DCBABB6449AA6D80 <@t> phx.gbl>
Content-Type: text/plain; charset="us-ascii"
Dear all,
I am using a Technovit 9100 PMMA embeding system and the pre-polymerisation
step requires a "partial vaccum". Partial is everyting which is not total
100% (Absolute) so the rage is quite wide.
Does any one using Technovit 9100 could tell which poucentage of vaccum to
apply to the sample or how many Inches of Mercury could be read on the
gauge?
Here is a link to web site if you are using an other unit to evalute your
vacuum.
http://www.engineeringtoolbox.com/vacuum-converter-d_460.html
Thank you for your help,
Jean-Philippe REY
(913) 213-2558
Jp1000r <@t> hotmail.com
------------------------------
Message: 3
Date: Mon, 12 Jan 2009 14:16:59 -0500
From: Kathleen Roberts <kgrobert <@t> rci.rutgers.edu>
Subject: [Histonet] Processing 3-dimensional cell cultures into
paraffin
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <496B972B.4010000 <@t> rci.rutgers.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Histonetters,
I just got a call from a researcher who is growing cells in soft agar in
3 dimensions and would like to process them into paraffin for
sectioning, which I have never done before. I know she wants to
preserve the 3D structure, and I found in the archives a protocol for
putting cell pellets into agar for subsequent processing into paraffin,
which gives me something to start with, sort of. Has anyone done this
before? Can this be done on a processor (I have a Sakura VIP 5), or
should I opt for manual processing? Either way, I would appreciate a
processing protocol if anyone has one.
Thanks so much,
Kathleen Roberts
Principal Lab Technician
Neurotoxicology Labs
Rutgers, the State University of NJ
------------------------------
Message: 4
Date: Mon, 12 Jan 2009 14:36:14 -0500
From: "Sherwood, Margaret " <MSHERWOOD <@t> PARTNERS.ORG>
Subject: [Histonet] Re: Automatic Stainer/coverslipper
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<073AE2BEA1C2BA4A8837AB6C4B943D9703E2372F <@t> PHSXMB30.partners.org>
Content-Type: text/plain; charset="us-ascii"
We are interested in purchasing an automatic stainer (and possible
coverslipper). The Leica ST5020 model was recommended to us. Is anyone
familiar with this stainer and/or Leica's CV5030 robotic coverslipper? The
price for both is quite high and I want to make sure people are satisfied with
it. We are a small research core lab whose histology needs are not as extensive
as a clinical histology lab.
Also, if anyone has another recommendation, I would be interested. Please reply
directly to me.
Thanks!
Peggy
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org
The information in this e-mail is intended only for the person to whom it is
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------------------------------
Message: 5
Date: Mon, 12 Jan 2009 15:12:12 -0600
From: Smith Wanda <Wanda.Smith <@t> HCAhealthcare.com>
Subject: RE: [Histonet] bluing in tap water?
To: Anne C Lewin <anne.lewin <@t> bms.com>, Eva Permaul
<eca9 <@t> georgetown.edu>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9E2D36CE2D7CBA4A94D9B22E8328A3BA9EFC4362 <@t> NADCWPMSGCMS03.hca.corpad.net>
Content-Type: text/plain; charset="us-ascii"
What should the pH of tap water be to blue just right and not too much???
WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC 29406
843-847-4586
843-847-4296 fax
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Anne C Lewin
Sent: Monday, January 12, 2009 12:04 PM
To: Eva Permaul
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] bluing in tap water?
When I have used tap water, I use cold running water for 5 minutes.
Works fairly well, depending on the pH of your tap water.
Eva Permaul wrote:
> Good morning,
> I was wondering if someone uses tap water to blue their slides after
> Hematoxyline. If yes, do you use warm or cold water and for how long?
> Thanks,
> Eva
> Georgetown University
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Tue, 13 Jan 2009 08:56:48 +1100
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] bluing in tap water?
To: "Smith Wanda" <Wanda.Smith <@t> HCAhealthcare.com>, "Anne C Lewin"
<anne.lewin <@t> bms.com>, "Eva Permaul" <eca9 <@t> georgetown.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <B9EAF61856077F47BF9BE2F89AFC555202FB01C9 <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="us-ascii"
I don't think you can blue too much.
If the pH is too high then it can bleach the haematoxylin.
Any mildly alkaline solution will do (in fact neutral tap water (pH 7)
will slowly get there.
The "special blueing" solutions available are many and varied:
Warm tap water, phosphate buffer (pH7-8), a weak sodium hydroxide
solution (< 0.5%), a lithium carbonate solution (saturated or a diluted
form), a few drops of ammonia in water, Scott's blueing solution, etc.
If you are worried about the alkalinity of your blueing solution check
it with some pH strips.
If the solution appears a slight pink (indicating that carry-over
haematoxylin is in its acidic state) then the pH will be acidic and need
replacing.
How can you tell if the Haematoxylin is blued? Check microscopically.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Smith
Wanda
Sent: Tuesday, 13 January 2009 8:12 AM
To: Anne C Lewin; Eva Permaul
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] bluing in tap water?
What should the pH of tap water be to blue just right and not too
much???
WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC 29406
843-847-4586
843-847-4296 fax
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Anne C
Lewin
Sent: Monday, January 12, 2009 12:04 PM
To: Eva Permaul
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] bluing in tap water?
When I have used tap water, I use cold running water for 5 minutes.
Works fairly well, depending on the pH of your tap water.
Eva Permaul wrote:
> Good morning,
> I was wondering if someone uses tap water to blue their slides after
> Hematoxyline. If yes, do you use warm or cold water and for how long?
> Thanks,
> Eva
> Georgetown University
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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------------------------------
Message: 7
Date: Mon, 12 Jan 2009 17:10:29 -0500
From: Jamie E Erickson <jamie.erickson <@t> abbott.com>
Subject: [Histonet] Rat and Mouse Bone histology methods and
equipment--HELP
To: "histonet histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<OF6BD98F09.D6941376-ON8525753C.00777F15-8525753C.0079CF37 <@t> abbott.com>
Content-Type: text/plain; charset="US-ASCII"
Hello Histonetter,
I hope someone out there in
histoland can help me. I am about to be thrust (kicking and screaming)
into the world of undecalcified bone histology and I need lots of help. My
goals for 2009 will involve sectioning/staining and doing Image analysis
on undecalcified rat and mouse bone samples.
I am not completely unfamiliar with these methods but I learn these
methods from some wise old, I mean experienced ladies that were
specialists in bone histology. These ladies have now since retired and I
am without any methods.
I work in a small (2 histologist, 1 pathologist) histolab that does
mouse and rat work and only decalcifed bone tissue. I have done plastic
work in the past but that was 10 years ago in a land far, far away...
If anyone out there could please refer me to a histology text books
or share methods that would be of great help.
If you do rat and mouse bone work and would be will to talk about
methods/stains/ embedding/grinding/plastic processing/ image analysis that
would be absolutely great.
Thanks for any help you can provide..
Jamie
_______________________________
Jamie Erickson
Sr. Research Associate II
Department: DSMP
Abbott Bioresearch Center
100 Research Drive
Worcester, MA 01605-4341
508-688-3134
FAX: 508-793-4895
e-mail: jamie.erickson <@t> abbott.com
------------------------------
Message: 8
Date: Mon, 12 Jan 2009 14:28:00 -0800
From: "Cathy Mayton" <cathy <@t> wasatchhisto.com>
Subject: [Histonet] lab closing, equipment for sale
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <AFCAF553C92049A39D06AAB566069595 <@t> shop1e2e996aa5>
Content-Type: text/plain; charset="iso-8859-1"
Fellow histonetters,
I am retiring and closing my lab March 31, 2009. All lab equipment and consumables will be sold. There is equipment for ground histology, 3 automated microtomes, 1 paraffin microtome, 2 6 foot fume hoods, all peripheral equipment and consumables. There are also 10 brand new, never been used D-profile tungsten-carbide knives. There is too much to mention but please feel free to email me direct at Cathy <@t> wasatchhisto.com for serious inquiries only.
Cathy Mayton
Wasatch Histo Consultants, Inc.
------------------------------
Message: 9
Date: Tue, 13 Jan 2009 14:56:13 +1300
From: "Tanya Fulton" <TanyaF <@t> adhb.govt.nz>
Subject: [Histonet] Commercial Haematoxylin
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<A66907C014FA1248AB1ECF4E86E4EA440561A6B4 <@t> EXCHANGE01.adhb.govt.nz>
Content-Type: text/plain; charset="iso-8859-1"
Can anyone recommend any commercial Harris' or Gills Haematoxylins? We are looking into changing to commercial from our ones made in house, so any help in deciding which ones to trial would be appreciated.
Tanya
LabPLUS
Auckland City Hospital
New Zealand
------------------------------
Message: 10
Date: Tue, 13 Jan 2009 07:20:05 -0600
From: "Swain, Frances L" <SwainFrancesL <@t> uams.edu>
Subject: RE: [Histonet] Commercial Haematoxylin
To: "Tanya Fulton" <TanyaF <@t> adhb.govt.nz>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<B46595D426846345AD00664F29B8B2A805FAF336 <@t> MAIL2.ad.uams.edu>
Content-Type: text/plain; charset=us-ascii
We use the hematoxylin's from PolyScientific R and D from BayTown New York.
They are excellent. We use Harris', Weigert's, Mayer's and Gill's. They are
reasonable in price and the quality is outstanding.
Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic Surgery
Center for Orthopaedic Research
Barton Research Building 2R28
4301 West Markham Street
Little Rock AR 72205
(501) 686-8739 PHONE
(501) 686-8987 FAX
swainfrancesl <@t> uams.edu email
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tanya Fulton
Sent: Monday, January 12, 2009 7:56 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Commercial Haematoxylin
Can anyone recommend any commercial Harris' or Gills Haematoxylins? We are
looking into changing to commercial from our ones made in house, so any help
in deciding which ones to trial would be appreciated.
Tanya
LabPLUS
Auckland City Hospital
New Zealand
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------------------------------
Message: 11
Date: Tue, 13 Jan 2009 08:45:14 -0500
From: Jack Ratliff <ratliffjack <@t> hotmail.com>
Subject: RE: [Histonet] Undecalcified bone in MMA and antigen
retrieval (Neil Hand?)
To: <perron.b <@t> wanadoo.fr>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU108-W27964E816D8174ADF90A3BAED90 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Benjamin,
How about trying Haupt's coated slides. I am not sure if these slides are now commercially available, but you can coat them yourself. In the past I have made the solution by scratch and also used the commercially prepared solution from Fisher. When you go to the Fisher Scientific website, the product will be listed under Harleco Gelatin Fixative #785-71 @ 500 mL.
This is a concentrate solution, so it will be best to aliquot a small amount into a beaker, gently warm in a lab use only microwave for 10-20 seconds or a hot plate until the solution is in a complete liquid state, freely flowing. Then cut it 1:1 with 50% EtOH to prepare the working solution. Place 1-2 smal drops on a slide and use another slide of the same width to gently move the solution across the specimen mounting area. Finally, let it dry flat for 5-10 minutes and then you are ready to use.
Let me know if you need anymore help!
Jack Ratliff
> From: perron.b <@t> wanadoo.fr> To: histonet <@t> lists.utsouthwestern.edu> Date: Mon, 12 Jan 2009 19:02:05 +0100> Subject: [Histonet] Undecalcified bone in MMA and antigen retrieval (Neil Hand?)> > Hi all,> > I'm encountering problems while trying to do antigen retrieval (AR) on undecalcified bone (rat jaw) sections embedded in methylmetracrylate (MMA). Sections fall off the slides while heated.> Section are 4?m thick, deposit on APES or gelatine coated slides, and heating is perform after total deplastification and rehydratation (I tried microwaves and warming bath in citrate buffer pH=6.0).> > Could some around here share theyre experience with AR on undecalcified bone sections and in particular tell me :> > -does section thickness as an impact on sections detachment while heating (I know some are performing thinner section, but 3-4?m is the thinner we are (in routine) able to do with rat jaw)> -is theyre a particular coating for slides that would better fit that what we are using (APES or gelatine). Someone suggest me Poly-l-lysine.> > All tips and advices from experimented in this field would be very helpfull.> > Benjamin Perron> Student (Master in Cell Biology)> EA2496> Montrouge> France> _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Tue, 13 Jan 2009 08:49:43 -0500
From: Jack Ratliff <ratliffjack <@t> hotmail.com>
Subject: RE: [Histonet] Technovit 9100 and partial vacuum
To: <jp1000r <@t> hotmail.com>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU108-W2769F4BD2E1A464197B2C1AED90 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
JP,
I believe that Technovit 9100 is pretty much a typical MMA formulation. If so, then I would add whatever vacuum you can safely add to the specimen. I use an MMA + DBP formulation (not a kit) and draw a vacuum @ -15 to -20 inHg.
Let me know if you need anymore help!
Jack Ratliff
> From: jp1000r <@t> hotmail.com> To: histonet <@t> lists.utsouthwestern.edu> Date: Mon, 12 Jan 2009 12:20:05 -0600> Subject: [Histonet] Technovit 9100 and partial vacuum> > Dear all,> > > > I am using a Technovit 9100 PMMA embeding system and the pre-polymerisation> step requires a "partial vaccum". Partial is everyting which is not total> 100% (Absolute) so the rage is quite wide.> > Does any one using Technovit 9100 could tell which poucentage of vaccum to> apply to the sample or how many Inches of Mercury could be read on the> gauge?> > > > Here is a link to web site if you are using an other unit to evalute your> vacuum.> > http://www.engineeringtoolbox.com/vacuum-converter-d_460.html> > > > > > Thank you for your help,> > > > > > Jean-Philippe REY> > (913) 213-2558> > Jp1000r <@t> hotmail.com> > > > _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 13
Date: Tue, 13 Jan 2009 14:25:38 +0000
From: rmweber113 <@t> comcast.net
Subject: [Histonet] Job opportunity NJ
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<011320091425.18829.496CA462000910600000498D2216549976CCCECE9D0A0D0A99039D <@t> comcast.net>
Content-Type: text/plain
I have an opening for a FT/PT or per diem histologist at a in office endoscopy laboratory in North New Jersey. This is a state of the art facility with new equipment that uses the latest in microwave specimen processing. We are seeking a experienced, ASCP certified histologist who is proficient in all aspects of GI specimen processing.
Please send resume to above email address or call 732 814-1170
No headhunters please.
Thank you
------------------------------
Message: 14
Date: Tue, 13 Jan 2009 14:30:24 +0000
From: "SARAH REEVES" <SARAH.REEVES <@t> ekht.nhs.uk>
Subject: [Histonet] Mechanism of Staining - Special Stains
To: "<SARAH REEVES" <SARAH.REEVES <@t> ekht.nhs.uk>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <496CA580020000B500003867 <@t> ekhgwia.ekht.nhs.uk>
Content-Type: text/plain; charset="us-ascii"
Does anyone know of a good mechanism of staining reference for special stains?
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Message: 15
Date: Tue, 13 Jan 2009 07:25:47 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Commercial Haematoxylin
To: histonet <@t> lists.utsouthwestern.edu, Tanya Fulton
<TanyaF <@t> adhb.govt.nz>
Message-ID: <239578.78507.qm <@t> web65711.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I was always very satisfied with Ricard Allan's hematoxylin.
Ren? J.
--- On Mon, 1/12/09, Tanya Fulton <TanyaF <@t> adhb.govt.nz> wrote:
From: Tanya Fulton <TanyaF <@t> adhb.govt.nz>
Subject: [Histonet] Commercial Haematoxylin
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, January 12, 2009, 8:56 PM
Can anyone recommend any commercial Harris' or Gills Haematoxylins? We are
looking into changing to commercial from our ones made in house, so any help in
deciding which ones to trial would be appreciated.
Tanya
LabPLUS
Auckland City Hospital
New Zealand
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------------------------------
Message: 16
Date: Tue, 13 Jan 2009 10:41:10 -0500
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: RE: [Histonet] Commercial Haematoxylin
To: <rjbuesa <@t> yahoo.com>, <histonet <@t> lists.utsouthwestern.edu>, "Tanya
Fulton" <TanyaF <@t> adhb.govt.nz>
Message-ID:
<5D64396A0D4A5346BEBC759022AAEAA52581EA <@t> ITSSSXM01V6.one.ads.che.org>
Content-Type: text/plain; charset="iso-8859-1"
Richard Allen's 7211 is the most like the old Harris that I have come across. They started to discontinue it several years. I almost had heart failure! I wasn't the only one I guess, because they still have it. I called them immediately tho! J:>)
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, January 13, 2009 10:26 AM
To: histonet <@t> lists.utsouthwestern.edu; Tanya Fulton
Subject: Re: [Histonet] Commercial Haematoxylin
I was always very satisfied with Ricard Allan's hematoxylin.
Ren? J.
--- On Mon, 1/12/09, Tanya Fulton <TanyaF <@t> adhb.govt.nz> wrote:
From: Tanya Fulton <TanyaF <@t> adhb.govt.nz>
Subject: [Histonet] Commercial Haematoxylin
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, January 12, 2009, 8:56 PM
Can anyone recommend any commercial Harris' or Gills Haematoxylins? We are looking into changing to commercial from our ones made in house, so any help in deciding which ones to trial would be appreciated.
Tanya
LabPLUS
Auckland City Hospital
New Zealand
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Confidentiality Notice:
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------------------------------
Message: 17
Date: Tue, 13 Jan 2009 07:51:15 -0800 (PST)
From: Jessica Piche <jessgrocki <@t> yahoo.com>
Subject: [Histonet] Excelsior ES Tissue Processor
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <709115.94009.qm <@t> web82001.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
?
Good Morning:
?
Our lab is looking into getting a new processor at some point!? We are looking at the Excelsior ES Tissue Processor from Thermo Scientific. We were wondering if any of you Histol labs out there are using this machine and if so how you like it? Have you had any problems with it, ie. mechanical?? Is it saving you money? and also is anyone using the machine with the xylene free option?
?
Thanks so much! Have a great day!
?
Jessica Piche-Grocki, HT(ASCP)
Waterbury Hospital
------------------------------
Message: 18
Date: Tue, 13 Jan 2009 11:25:51 -0500
From: "Hamilton, Melissa" <mhamilton <@t> GENVEC.com>
Subject: [Histonet] X-Gal staining
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<223C48015FDAC04E884941EBDB2F639C06DFB0 <@t> genexch.genvec.com>
Content-Type: text/plain; charset="us-ascii"
I am interested in any suggestions for what mouse tissue organ and route
of administration that I could use as a postitive x-gal staining control
to tease out tissue processing, staining and paraffin embedding
artifacts.
Regards,
MH
------------------------------
Message: 19
Date: Tue, 13 Jan 2009 10:51:44 -0600
From: "Cheryl" <tkngflght <@t> yahoo.com>
Subject: RE: [Histonet] Commercial Haematoxylin
To: "'Weems, Joyce'" <JWeems <@t> sjha.org>, <rjbuesa <@t> yahoo.com>,
<histonet <@t> lists.utsouthwestern.edu>, "'Tanya Fulton'"
<TanyaF <@t> adhb.govt.nz>
Message-ID: <AAD246B9654C43189C6604FF3C05405C <@t> FULLSTAFF.ORG>
Content-Type: text/plain; charset="iso-8859-1"
We also always liked the Anatech version. Lasted forever, LOTS of heme in
the solution so filtering didn't cause a problem---it's been a while since
I've used it myself so you may want additional input. The only time we had
trouble was when it sat on the loading dock and froze and thawed a couple of
times before we got it---it took a while to figure it out but was easily
remedied once we did.
Hope this helps...
Cheryl
Cheryl R. Kerry, HT(ASCP), BA
Full Staff Inc.
Staffing Healthcare Professionals - One GREAT fit at a time.
281.913.7285 office
281.913.7288 fax hard line
281.883.7704 cell
800.756.3309 electronic fax and alternate phone
admin <@t> fullstaff.org
www.fullstaff.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Tuesday, January 13, 2009 9:41 AM
To: rjbuesa <@t> yahoo.com; histonet <@t> lists.utsouthwestern.edu; Tanya Fulton
Subject: RE: [Histonet] Commercial Haematoxylin
Richard Allen's 7211 is the most like the old Harris that I have come
across. They started to discontinue it several years. I almost had heart
failure! I wasn't the only one I guess, because they still have it. I called
them immediately tho! J:>)
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, January 13, 2009 10:26 AM
To: histonet <@t> lists.utsouthwestern.edu; Tanya Fulton
Subject: Re: [Histonet] Commercial Haematoxylin
I was always very satisfied with Ricard Allan's hematoxylin.
Ren? J.
--- On Mon, 1/12/09, Tanya Fulton <TanyaF <@t> adhb.govt.nz> wrote:
From: Tanya Fulton <TanyaF <@t> adhb.govt.nz>
Subject: [Histonet] Commercial Haematoxylin
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, January 12, 2009, 8:56 PM
Can anyone recommend any commercial Harris' or Gills Haematoxylins? We are
looking into changing to commercial from our ones made in house, so any help
in deciding which ones to trial would be appreciated.
Tanya
LabPLUS
Auckland City Hospital
New Zealand
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice:
This email, including any attachments is the property of Catholic Health
East and is intended for the sole use of the intended recipient(s).
It may contain information that is privileged and confidential. Any
unauthorized review, use, disclosure, or distribution is prohibited. If you
are not the intended recipient, please reply to the sender that you have
received the message in error, then delete this message.
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------------------------------
Message: 20
Date: Tue, 13 Jan 2009 17:02:51 +0000
From: anh2006 <@t> med.cornell.edu
Subject: Re: [Histonet] X-Gal staining
To: "Hamilton, Melissa" <mhamilton <@t> GENVEC.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<669371962-1231866316-cardhu_decombobulator_blackberry.rim.net-1085777367- <@t> bxe277.bisx.prod.on.blackberry>
Content-Type: text/plain
The mouse epididymus has endogenous activity.
However I suggest you use an animal transgenic for lacZ (any organ from a Rosa26 mouse for example).
Sorry I do not understand what ypu mean by route of administration.
------Original Message------
From: Hamilton, Melissa
Sender: histonet-bounces <@t> lists.utsouthwestern.edu
To: histonet <@t> lists.utsouthwestern.edu
Sent: Jan 13, 2009 11:25 AM
Subject: [Histonet] X-Gal staining
I am interested in any suggestions for what mouse tissue organ and route
of administration that I could use as a postitive x-gal staining control
to tease out tissue processing, staining and paraffin embedding
artifacts.
Regards,
MH
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 21
Date: Tue, 13 Jan 2009 17:16:43 +0000
From: anh2006 <@t> med.cornell.edu
Subject: Re: [Histonet] bluing in tap water?
To: "Smith Wanda" <Wanda.Smith <@t> HCAhealthcare.com>, "Anne C Lewin"
<anne.lewin <@t> bms.com>, "Eva Permaul" <eca9 <@t> georgetown.edu>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<2022117152-1231867149-cardhu_decombobulator_blackberry.rim.net-1686426943- <@t> bxe277.bisx.prod.on.blackberry>
Content-Type: text/plain
Is there such a thing as bluing too much?
-----Original Message-----
From: Smith Wanda <Wanda.Smith <@t> HCAhealthcare.com>
Date: Mon, 12 Jan 2009 15:12:12
To: Anne C Lewin<anne.lewin <@t> bms.com>; Eva Permaul<eca9 <@t> georgetown.edu>
Cc: histonet <@t> lists.utsouthwestern.edu<histonet <@t> lists.utsouthwestern.edu>
Subject: RE: [Histonet] bluing in tap water?
What should the pH of tap water be to blue just right and not too much???
WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC 29406
843-847-4586
843-847-4296 fax
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Anne C Lewin
Sent: Monday, January 12, 2009 12:04 PM
To: Eva Permaul
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] bluing in tap water?
When I have used tap water, I use cold running water for 5 minutes.
Works fairly well, depending on the pH of your tap water.
Eva Permaul wrote:
> Good morning,
> I was wondering if someone uses tap water to blue their slides after
> Hematoxyline. If yes, do you use warm or cold water and for how long?
> Thanks,
> Eva
> Georgetown University
>
>_______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 22
Date: Tue, 13 Jan 2009 11:37:02 -0600
From: "Parker, Helayne" <HParker <@t> Skaggs.Net>
Subject: [Histonet] Spit and Diastase
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<2FAF8CC41C5AAF43AAA52F26782E1A5602610FC6 <@t> mail1-schc.skaggs.net>
Content-Type: text/plain; charset="us-ascii"
Hi Gang,
What would cause someone's spit to no longer work while doing a PAS
w/ Diatase. She is a trainee and has done this several times prev to
this w/o any issues and all the sudden her spit does not work. Did not
work yesterday nor did the repeat today work. Well off to repeat it
again w/ my spit.
Any suggestions,
Thanks
Helayne Parker
------------------------------
Message: 23
Date: Tue, 13 Jan 2009 12:46:15 -0500
From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
Subject: RE: [Histonet] Processing 3-dimensional cell cultures into
paraffin
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4EBFF65383B74D49995298C4976D1D5E03835C83 <@t> LSRIEXCH1.lsmaster.lifespan.org>
Content-Type: text/plain; charset="iso-8859-1"
I have processed and embedded cells grown in/on agar, or post-embedded in agar or agarose, many times. It really isn't much different from processing and embedding tissue. You can trim the agar blocks to appropriate size with a scalpel (if necessary) either before or after fixation (if you trim them before fixation, do it while they are cold, so the agar is firm), and place them in a cassette just like a piece of tissue. Put them on the processor as usual, processing times the same as you would use for tissues of equivalent size, and embed as usual. One difference in sectioning however. Don't place the blocks in water after facing them off. The agar absorbs too much water and becomes soft. The blocks should be cut cold, but not moistened. Either place them on a cold plate without water, or put them in the refrigerator after facing, and take them out one at a time as you section them.
------------------------------
Message: 24
Date: Tue, 13 Jan 2009 12:49:08 -0500
From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" <jqb7 <@t> cdc.gov>
Subject: RE: [Histonet] Processing 3-dimensional cell cultures into
paraffin
To: "Monfils, Paul" <PMonfils <@t> Lifespan.org>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<1CE1847DFEA0A647B1CCDE4108EA60A70402AD8C <@t> LTA3VS011.ees.hhs.gov>
Content-Type: text/plain; charset=us-ascii
Histogel works great and does not absorb water like plain agar does.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Monfils,
Paul
Sent: Tuesday, January 13, 2009 12:46 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Processing 3-dimensional cell cultures into
paraffin
I have processed and embedded cells grown in/on agar, or post-embedded
in agar or agarose, many times. It really isn't much different from
processing and embedding tissue. You can trim the agar blocks to
appropriate size with a scalpel (if necessary) either before or after
fixation (if you trim them before fixation, do it while they are cold,
so the agar is firm), and place them in a cassette just like a piece of
tissue. Put them on the processor as usual, processing times the same as
you would use for tissues of equivalent size, and embed as usual. One
difference in sectioning however. Don't place the blocks in water after
facing them off. The agar absorbs too much water and becomes soft. The
blocks should be cut cold, but not moistened. Either place them on a
cold plate without water, or put them in the refrigerator after facing,
and take them out one at a time as you section them.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 25
Date: Tue, 13 Jan 2009 12:49:13 -0500
From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
Subject: RE: [Histonet] Spit and Diastase
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4EBFF65383B74D49995298C4976D1D5E03835C84 <@t> LSRIEXCH1.lsmaster.lifespan.org>
Content-Type: text/plain; charset="iso-8859-1"
Is she doing her PAS stains right after lunch?
------------------------------
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End of Histonet Digest, Vol 62, Issue 15
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