[Histonet] Antigen Retrieval on MMA slides

Nancy W. Troiano nancy.troiano <@t> yale.edu
Tue Jan 13 12:32:12 CST 2009


We put our plastic sections (5-6 microns in thickness) onto slides 
manufactured by Scientific Device Laboratories, the "in situ plus" 
slides.  We then bake the sections overnight in a 60 degree oven, then 
bring to room temperature before doing immunostaining.  Sections will not 
stay on gel coated slides and the gelatin on the slides will interfere with 
your immunostaining.




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