[Histonet] Processing 3-dimensional cell cultures into paraffin

[Kathleen Roberts]

Histonetters,

I just got a call from a researcher who is growing cells in soft agar in 
3 dimensions and would like to process them into paraffin for 
sectioning, which I have never done before.  I know she wants to 
preserve the 3D structure, and I found in the archives a protocol for 
putting cell pellets into agar for subsequent processing into paraffin, 
which gives me something to start with, sort of.  Has anyone done this 
before?  Can this be done on a processor (I have a Sakura VIP 5), or 
should I opt for manual processing?  Either way, I would appreciate a 
processing protocol if anyone has one.

Thanks so much,
Kathleen Roberts
Principal Lab Technician
Neurotoxicology Labs
Rutgers, the State University of NJ