[Histonet] bluing in tap water?

[Paula Pierce]

Hello,

I rinse in tap water, but blue in 1-2% ammonium hydroxide for ~ minute, then rinse again in tap water.

Paula




________________________________
From: Eva Permaul <eca9 <@t> georgetown.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Monday, January 12, 2009 8:35:15 AM
Subject: [Histonet] bluing in tap water?

Good morning,
I was wondering if someone uses tap water to blue their slides after 
Hematoxyline. If yes, do you use warm or cold water and for how long?
Thanks,
Eva
Georgetown University

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