[Histonet] Re: E16 paraffin embedding

[Jennifer Springsteen]

When we process embryos this old, the are so large and their skin is so 
thick that we have to make a long slit down their back with a razor 
blade to allow infiltration.  Otherwise, we get a soft, mushy mess that 
can't be sectioned.  Depending on what you are interested, you could 
them in half down the midline, or do a slit down the back or belly.  
Good luck!

-- 
Jennifer L. Springsteen, B.S.
Lab Manager, Assistant Scientist
Lillehei Heart Institute Histology & Microscopy Core Facility
  University of Minnesota School of Medicine, Division of Cardiology
  312 Church Street SE
  4-266 Nils Hasselmo Hall
  Minneapolis, MN  55455
Lab: 612-626-3090
  Cell: 651-357-7916
  Fax: 612-624-8118