[Histonet] E16 paraffin embedding

[James Dooley]

Hello All,

I
have been having trouble cutting whole embryo sections on E16 mice. I
think the problem is with the fixation process. I am currently using a
modified Carnoy's fixation (60% ETOH, 30% formaldehyde, 10% Glacial
acetic acid). I would like to use this fixation as I have optimized my
antibodies for this condition. The tissue is soft. I think it is due to
incomplete dehydration. Does anyone have a any suggestions for doing
tissue this large as I know over dehydrating can lead to problems also. 

Thank you,
James