[Histonet] H&E help
Bryan Llewellyn
llewllew <@t> shaw.ca
Fri Jan 9 10:01:52 CST 2009
Mayer's hemalum is a nuclear selective progressive formula so the staining
is largely confined to nuclei. If the pathologists want more blue tinge in
other parts of the tissue, then you should change the hemalum, or simply
increase the hematoxylin content in the solution. Cole's does stain darker
than Mayer's, but has 1.5 grams hematoxylin per litre, fully oxidised. Try
that. You might want to decrease the time in eosin as well. Try one
minute.
----- Original Message -----
From: "Charles, Roger" <rcharles <@t> state.pa.us>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, January 09, 2009 6:13 AM
Subject: [Histonet] H&E help
Hello All,
TGIF!!!!
I'm trying to help out one of my veterinarian pathologist in getting some
information on how to increase the bluing of the H&E staining. Presently we
are using pre made Mayers Hematoxylin from Sigma with an automated schedule
as follows:
1. Drying 45min
2. citrisolve 3 min
3. citrisolve 30 sec
4. citrisolve 3min
5. 100% 45 sec
6. 100% 45 sec
7. 100% 3 min
8. 95% 30 sec
9. 95% 1 min 30 sec
10. Tap water 1 min
11. Hematoxylin 8 min
12. tap water 4 min
13. eosin 1 min 30 sec
14. 95% 1 min
15. 95% 1 min
16. 95% 1 min
17. 100%1 min
18. 100%1 min
19. 100%1 min
20. Citrisolve 1 min
21. Citrisolve 1 min
22. Citrisolve 1 min
23. Citrisolve up to 120 min
The pathologist is stating the slides are too eosinic and would like greater
bluing. My question is "are there limitations to the Mayers Hematoxylin on
how blue it will actually get and is there something else we can change in
our schedule to increase the bluing or decrease the eosin to get the desired
affect?
Thanks to all
Roger Charles
Microbiologist
Pennsylvania Veterinary Laboratory
2305 N Cameron St
Harrisburg, PA 17110
717-787-8808
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
More information about the Histonet
mailing list