[Histonet] Cryostat safety question

[anh2006 <@t> med.cornell.edu]

That sounds very cool for the lucky owners of one of your cryostats, esp the part about orientation ... but nearly every block of ours goes back in the freezer so I think it would take up too much space and end up being expensive, no (we are talking 100s and 100s of block)?

------Original Message------
From: Alan Bright
To: Andrea Hooper
To: Histonet
Sent: Jan 8, 2009 7:06 AM
Subject: RE: [Histonet] Cryostat safety question

Andrea,

For users of our cryostats that need to use the specimen for future use
we manufacture  disposable object holders & quick release holders that
fits specimen containers for LN2 or -80 deg. C storage. The tissue stays
on the disposable object holders during storage too and has an index
location so that it only goes back onto the cryostat microtome in the
correct orientation. No sorry but it will not be suitable for your
cryostat or other Chinese/ German manufactured types.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright <@t> brightinstruments.com
Web Site: www.brightinstruments.com
Skype: dazzle0




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Andrea
Hooper
Sent: 07 January 2009 23:40
To: Histonet
Cc: mari.ann.mailhiot <@t> leica-microsystems.com
Subject: [Histonet] Cryostat safety question

The discussion on microtome safety begs me to ask a cryostat question
....

We have a Leica CM3050 cryostat and love it!

How are people (and perhaps only those in research do this) removing 
their tissue from the chucks for future use? We often just section a 
few slides worth then put the block at -80 deg C for future studies. 
Needless to say, it's the most dangerous part of our day.

So what are your suggestions for removing tissue from a chuck (and 
melting it isn't really a viable option)?

Thanks in advance,
Andrea
-- 

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