[Histonet] Microtome Cutting Safety

Bartlett, Jeanine (CDC/CCID/NCZVED) jqb7 <@t> cdc.gov
Wed Jan 7 06:25:32 CST 2009


I use my fingers too, so no help there.

But an automated microtome is great because it frees both hands to
handle a ribbon as it is coming off the blade.  It is easy to get
careless if you use a foot pedal but the one time I have been seriously
cut it was with a manual microtome so I guess the moral is to simply be
careful.


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartlett <@t> cdc.hhs.gov


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Donna
Harclerode
Sent: Tuesday, January 06, 2009 11:43 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Microtome Cutting Safety



Begin forwarded message:

> From: Donna Harclerode <eridana <@t> cox.net>
> Date: January 6, 2009 8:27:14 PM PST
> To: histonet-request <@t> lists.utsouthwestern.edu
> Subject: Microtome Cutting Safety
>
> Anyone know of a paraffin microtome that you can section WITH the 
> knife guard in position? I have always used forceps to keep my fingers

> safe, but some techs are harder to convince to do this . I figured if 
> anyone knows they would be on this list.
> I tried the Leica RM2255 (both in automated and manual mode) and I 
> could sort of section a couple small blocks with the guard up, but it 
> was not going to work with anything except perfect processed small 
> blocks and not well for those.
>
> Any opinions on the safety automated versus manual microtomes?  I 
> adore  automated cryostats (Leica 3050 is my favorite) , but I can not

> figure why use an automated paraffin microtome. Logically I always 
> figured an automated can do more damage, but really have no facts.
>
> Thanks in advance,
>
> Donna Harclerode, HT, HTL, SLS, (ASCP) QIHC

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