[Histonet] Differences between WB antibody and IHC antibody

[Patsy Ruegg]

As Ray states it is all empirical, some will work from WB to IHC and some
will not in my experience.  In general if the spec sheet recommends a
dilution for WB at say 1:2000, I will start using the ab for IHC on tissue
at 1:200 (it will definitely need to be tittered and used at probably around
10 fold more concentrated for IHC on tissue).  I like getting info on the ab
for WB and sometimes that may be all you get.

Best regards,
Patsy


Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
email pruegg <@t> ihctech.net
website www.ihctech.net
IHC Resource Group www.ihcrg.org 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Friday, January 02, 2009 12:26 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Differences between WB antibody and IHC antibody

If anyone replies with posting to the list, please forward it.  I've always
wondered the same thing.

On Fri, Jan 2, 2009 at 8:15 AM, TF <tifei <@t> foxmail.com> wrote:

> Hi
> Just wondering whether the antibody for WB (only WB, IP on datasheet) can
> be used for IHC use?
> If not, why?
>
> Can I just increase the concentration for IHC application?
>
> I want to learn more about the underlying production processes.
> Thanks!
>
>
> 2009-01-02
>
>
>
> TF
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