[Histonet] IHC automated stainers

[Kipp Greicar]

 Is anyone using either the biogenics xmatrix or the biocare intellipath?

I am shopping and could sure use some feedback.

Thanks

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Today's Topics:

   1. Differences between WB antibody and IHC antibody (TF)
   2. IgM monoclonal antibody compatibility (anjan kumar)
   3. 2009 RVU Values (Nita Searcy)


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Message: 1
Date: Fri, 2 Jan 2009 21:15:00 +0800
From: "TF" <tifei <@t> foxmail.com>
Subject: [Histonet] Differences between WB antibody and IHC antibody
To: "Anatoli Gleiberman" <AGleiberman <@t> cbiolabs.com>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200901022114549441081 <@t> foxmail.com>
Content-Type: text/plain;	charset="gb2312"

Hi
Just wondering whether the antibody for WB (only WB, IP on datasheet) can be
used for IHC use?
If not, why?

Can I just increase the concentration for IHC application?

I want to learn more about the underlying production processes.
Thanks!


2009-01-02 



TF 


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Message: 2
Date: Fri, 2 Jan 2009 21:59:38 +0530
From: anjan kumar <drvet_anjan <@t> hotmail.com>
Subject: [Histonet] IgM monoclonal antibody compatibility
To: triple immunohistochem <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU149-W42F4FC43DF4B127D5707478BE20 <@t> phx.gbl>
Content-Type: text/plain; charset="Windows-1252"


hi,
  i wanted to  know if there are any difference in selecting secondary
antibody against IgM compared to secondary antibody for IgG.
as i was using CD24 which is a mouse monoclonal IgM, what kind of secondary
antibody should i select, currently i m hoping that goat anti mouse will
work.
can any one plz suggest me on this.

Regards,
Dr. Anjan Kumar
Junior Research Scientist

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Message: 3
Date: Fri, 02 Jan 2009 10:38:49 -0600
From: "Nita Searcy" <NSEARCY <@t> swmail.sw.org>
Subject: [Histonet] 2009 RVU Values
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <495DEEB9020000EF0001323E <@t> MERCURY.SW.ORG>
Content-Type: text/plain; charset=US-ASCII

Anyone out there received the new values for 2009 that start today?

Thanks

Nita




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