[Histonet] Gills 3 Hematoxylin
Va Paula Sicurello
vapatpxs <@t> yahoo.com
Fri Feb 27 17:23:46 CST 2009
Hi Histo People,
I tried to H&E with a Vector Lab's Gill's Formulation of Hematoxylin. Everything E'd just fine but there is no H.
Is there a way to go back and re-H after coverslipping?
As a clarifier I used 1% glacial acetic acid for 20 seconds and blueing was saturated Lithium carbonate.
I am using Gill's of unknown age (for 1 minute), the slides seemed sort of light after the water rinses but I went on thinking it would blue later. Nope-there is no nuclear stain at all.
Can I go back and fix this? Remove coverslips, rehydrate and re-Hematoxylin?
Please give me any suggestions you can think of.
These blocks were a pain in the rear to section and I'd hate to have to resection them.
Boo-Hoo & Waaah,
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Microscope Facility, room B141
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
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