[Histonet] Re: Brazilliant

[Ada Feldman]

For general information:

Brazilliant is the product name for Anatech's red nuclear stain  
solution. It is available and will continue to be available for the  
foreseeable future. The dye brazilin used to prepare Brazilliant also  
will continue to be available from Anatech.



Ada T. Feldman
ANATECH LTD.
1020 Harts Lake Road
Battle Creek, MI 49037

Phone: 800.262.8324
Fax: 269.964.8084
email: adafeldman <@t> anatechltdusa.com
website: www.anatechltdusa.com




On Feb 26, 2009, at 11:42 PM, Robert Richmond wrote:

> Sarah Jones at Dako responds - and Sarah, please forgive me for
> quoting you onto Histonet, but this seems to me to be a public
> message:
>
>> Brazilliant will soon not be available for some time.  The  
>> government in the
>> state of Pernambuco in Brazil has declared a 10 year moratorium on  
>> the
>> felling of the Pernambuco trees from which this dye is made.  This  
>> took
>> place in September of 2007, if I remember correctly.  Violin bows  
>> are also
>> made from this tree.  I was doing some work here at Dako trying to  
>> use it in
>> place of Nuclear Fast Red.  But when I learned of this moratorium  
>> (at the
>> Biological Stain Commission Annual Meeting in June of 2008), I  
>> stopped
>> working on it.  I believe I sent you some of my photomicrographs  
>> taken at
>> that time.  I never heard back from you, so I thought you lost  
>> interest.  I
>> can send them to you again if you want me to.  Just let me know.
>
> And Allen Smith responded:
>
>> I have used brazalum, made by substituting brazilin for  
>> hematoxylin in the recipe for Mayer's hemalum. It stains nuclei a  
>> slightly deeper red than nuclear fast red.  Colorfastness is  
>> excellent.  I have 40-year old slides stained with brazalum that  
>> still look as they did on the day I made them.
>> Allen A. Smith, Ph.D.
>> Barry University School of Podiatric Medicine
> **********************
> I think this clarifies the problem. I would hope that something could
> be worked out with the Brazilian government for the very small amount
> of brazil wood histologists require, but the Brazilian government has
> been difficult about issues like this - see a number of citations in
> Science over the last few years.
>
> Sarah, I was much impressed by your photomicrographs of stains with
> brazalum nuclear counterstaining, and I apologize for having failed to
> reply earlier.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
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