[Histonet] IHC staining on decalcified tissue. .

[Henry, Charlene]

I have a single protocol for each antibody; but on this protocol I address each possible fixative and decalcification variable that may be requested with the particular antibody (formalin, B+, EDTA decalcification, formic acid decalcification, HCL decalcification etc). Sometimes the protocol remains the same for all variables but most of the time I will need a different pretreatment time and/or antibody dilution/incubation time. Hope this helps.
Charlene



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Monday, February 23, 2009 1:35 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC staining on decalcified tissue. .

Does anyone have a different IHC staining protocol and/or modification for specimens that have been decalcified?  CAP asks on the IHC checklist if the "procedure manual includes appropriate modifications to address such specimens."



Laurie Colbert

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