[Histonet] Sanderson's Rapid Bone stain

[Suzanne Bruce]

The tissue in plastic will be rabbit femurs I believe.  We haven't started anything yet, just getting references.  As to the paraffin staining, I believe that was an overall question from my director to see if she could incorporate that stain in paraffin as well as our Technovit 7200 work.
 

________________________________

From: Jack Ratliff [mailto:ratliffjack <@t> hotmail.com]
Sent: Wed 2/18/2009 5:24 PM
To: Suzanne Bruce; Histonet
Subject: RE: [Histonet] Sanderson's Rapid Bone stain


What is the tissue? What are your endpoints?
 

 
> Date: Wed, 18 Feb 2009 16:45:46 -0500
> From: sbruce <@t> vetpathservicesinc.com
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Sanderson's Rapid Bone stain
> 
> Hello, I posted earlier regarding the Sanderson's Rapid Bone stain. I forgot to mention that I will be using this stain on 75-100 micron thick ground sections embedded in Technovit 7200 plastic. 
> 
> I am also wondering about paraffin staining using this stain.
> 
> Here's one response so far:
> "I've used Sanderson's on plastic sections a lot in the past, works well. Recently I tried it on rat tibias, decaled, FFPE. Very disappointing. They look like Toluidine blue-- all shades of blue & nothing else. Now I just stick with von kossa and/or tetrachrome for those.
> 
> Kari"
> 
> Any advice is welcome. 
> Suzanne
> 
> _______________________________
> Suzanne Bruce, R.V.T.
> Histologist / Necropsy Coordinator
> Vet Path Services, Inc. (VPS)
> 6450 Castle Dr.
> Mason, OH 45040
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> Email: sbruce <@t> vetpathservicesinc.com
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