[Histonet] Sanderson's Rapid Bone Stain

[Jack Ratliff]

Suzanne,

 

I use this stain two ways routinely for "thick" or "ground" sections (35-50 microns) embedded in MMA & DBP. You can stain with SRBS alone @ RT for 3-5 minutes after a brief acid etch in 0.7% FA for 30 sec (rinse in DI water and blot dry) or combine it with a Van Gieson picrofuchsin counterstain for 1-2 minutes (rinse in 100% EtOH and blot dry) after staining first in SRBS @ 60C for 10 minutes (rinse in DI water @ 60C and blot dry).

 

I have never used or needed to use SRBS on "thin" plastic sections because I routinely use MacNeals tetrachrome combined with Von Kossa and a Modified Goldner's to obtain all my bone related information. With that said, I have also never used this stain on paraffin sections and would be interested in the results if you try it.

 

Jack Ratliff


 
> Date: Wed, 18 Feb 2009 07:29:39 -0500
> From: sbruce <@t> vetpathservicesinc.com
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Sanderson's Rapid Bone Stain
> 
> We are planning on trying this stain on our plastics. Has anyone tried it on paraffin slides? If so, what were the results?
> 
> Thanks in advance for any help.
> Suzanne
> 
> _______________________________
> Suzanne Bruce, R.V.T.
> Histologist / Necropsy Coordinator
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet