[Histonet] semen analysis

[Anne van Binsbergen]

thanks for the email
we are a hospital based diagnostic lab and currently do all the testing
manually

i am actually asking what EQUIPMENT is being used for AUTOMATED analysis
(count, conc, motility etc)

regards
Anne

2009/2/15 Malcolm McCallum <Malcolm.McCallum <@t> tamut.edu>

>  semen analysis can be done using various methods depending on what you
> are analyzing.
> I am not certain if you are asking for techniques, or specific procedures.
> However, fluorescence microscopy can be used to assess simple morphology
> using mitotracker blue.
> Fluorescence can also be used with a fast green (I think this is the other
> dye) and propidium iodide to assess viability.
> alternatively, you can assess viability using flow cytometry with the same
> dyes.
> Also, morphology is typically done using SEM and TEM.
> there are several techiques for assessing motility.
>
> Jill Jenkins at the USGS National Wetlands Laboratory in Lafayette, LA does
> the flow cytometry.
> Anne Grippo at Arkansas State University uses the propidium iodide
> technique,
> Stan Trauth does the sperm morphology with EM and FM.
>
> Also, the Dallas Zoo has specialized instrumentation specifically for sperm
> work, but the name of the instrument
> has slipped my mind!
>
> They are doing this on non-human animals, so I'm not sure how applicable
> this is to your question!
>
> Malcolm L. McCallum
> Associate Professor
> Department of Biological Sciences
> Texas A&M University Texarkana
> 2600 Robison Rd.
> Texarkana, TX 75501
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> H: 1-903-791-3843
> Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html
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> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Anne van
> Binsbergen
> Sent: Wed 2/11/2009 6:45 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] semen analysis
>
> who out there is using automation for semen analysis and what equipment is
> recommended?
> i am considering the SQA-V from MES
> what say you?
>
> cheers
>
> --
> Anne van Binsbergen (Hope)
> Abu Dhabi
> UAE
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-- 
Anne van Binsbergen (Hope)
Abu Dhabi
UAE