[Histonet] RE:CD4 on Ventana

Isaac Milos imilos <@t> cellmarque.com
Thu Feb 12 16:10:35 CST 2009


Sorry for the delay on the response here.  ;-)

We actually now have an IVD rabbit monoclonal CD4 that performs
exceptionally well on the Ventana platform (as well as every other
platform that it has been tried on).

In the past, we tried to develop the other mouse monoclonals (such as
the 1F6) ourselves but they did not perform to our expectations.  The
sensitivity was far too low.  These issues were remedied when we
developed the rabbit monoclonal version.

If you are interested, additional information can be found at:

Best regards,

Isaac Milos
Technical Consultant
800.665.7284x8991(toll free)
360.981.6505 (mobile)
916.746.8989 (fax)

Message: 19
Date: Fri, 7 Nov 2008 20:15:11 +1100
From: "Piero Nelva" <pieronelva01 <@t> bigpond.com>
Subject: [Histonet] CD4 on Ventana
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <2D0669F5E5EA4786A17AE07D652FFF51 <@t> pentium4>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";

Dear Histonetters

I'm having a lot of trouble getting CD4 to stain FFPE tissue using the 
Ventana Benchmark XT.  Any advice about which antibody and protocol 
suggestions would be greatly appreciated.

Piero Nelva
Anatomical Pathology
Monash Medical Centre

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