[Histonet] Manual IHC for H. pylori

godsgalnow <@t> aol.com godsgalnow <@t> aol.com
Thu Feb 12 10:10:50 CST 2009

How manual?? DO you have a pressue cooker?

Retrieve in Diva (available from Biocare) in pressure cooker
Cool for 10 minutes
Buffer for 5 minutes
Rinse in DI water
H202 for 5 minutes
Buffer rinse
Background Sniper for 10 Minutes (also from Bioacre)
Buffer rinse
Hpylori antibody (1:150 titer--purchased from Biocare) for 30 minutes
Buffer rinse
Mach2 Alk Phose detection (Biocare)?for 30 minutes
Buffer Rinse
VFR (Biocare) for 15 minutes
Buffer rinse
Di water rinse
dry in oven
dip in xylene one slide at a time as coverslipping

Beautiful....if you have any questions, be glad to help


-----Original Message-----
From: Rosa Fields <rfields <@t> gidocs.net>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Thu, 12 Feb 2009 10:48 am
Subject: [Histonet] Manual IHC for H. pylori

Anyone have a manual IHC protocol for H. pylori they would be willing to share?  

Rosa Fields, HT (ASCP)
Gastroenterology Specialties
Histology Supervisor
4545 R Street
Lincoln, NE  68503
rfields <@t> gidocs.net

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