[Histonet] Manual IHC for H. pylori

[godsgalnow <@t> aol.com]

How manual?? DO you have a pressue cooker?

Dewax
Retrieve in Diva (available from Biocare) in pressure cooker
Cool for 10 minutes
Buffer for 5 minutes
Rinse in DI water
H202 for 5 minutes
Buffer rinse
Background Sniper for 10 Minutes (also from Bioacre)
Buffer rinse
Hpylori antibody (1:150 titer--purchased from Biocare) for 30 minutes
Buffer rinse
Mach2 Alk Phose detection (Biocare)?for 30 minutes
Buffer Rinse
VFR (Biocare) for 15 minutes
Buffer rinse
Di water rinse
dry in oven
dip in xylene one slide at a time as coverslipping

Beautiful....if you have any questions, be glad to help

Roxanne


-----Original Message-----
From: Rosa Fields <rfields <@t> gidocs.net>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Thu, 12 Feb 2009 10:48 am
Subject: [Histonet] Manual IHC for H. pylori




Anyone have a manual IHC protocol for H. pylori they would be willing to share?  



Rosa Fields, HT (ASCP)
Gastroenterology Specialties
Histology Supervisor
4545 R Street
Lincoln, NE  68503
402-465-4545
rfields <@t> gidocs.net

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