Re: [Histonet] Need some staining help

[TF]

hi, for normal brain sections at 40 um
either floating or on slides

1. dry up
2. rehydration before IHC
3. citrate buffer 95 C 30 min (increase the signal)
4. wash with PBS
5. 2N HCl 30 min @ 37C (4N HCl 20 min RT does not need citrate buffer step)
6. wash with PBS or borate buffer
7. anti BrdU.....




2009-02-12 



TF 



发件人： Jennifer Sipes 
发送时间： 2009-02-12  21:50:19 
收件人： histonet 
抄送： 
主题： [Histonet] Need some staining help 
 
I have an investigator that is doing BRDu (?) staining and is having trouble with it.?Does anyone out there have a protocol that works well so we can compare it with ours?
Thanks a bunch!
Jennifer Sipes 
Sr. Laboratory Technician 
Johns Hopkins University 
Ross 929 and Ross 933
720 Rutland Avenue 
Baltimore, MD?21205 
phone:?410-614-0131 
牋牋牋牋牋牋牋 410-955-9688 
fax:牋牋牋牋 410-955-9677 
cell:牋牋牋?443-631-6361 
e-mail:?jsipes1 <@t> jhmi.edu ?
      
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