[Histonet] Sections coming off of slides

[Kathleen Roberts]

Oh, I do know that is the problem-the slides that were used in the first 
place.  The other problem is, I wasn't the one who cut these; it was 
someone in a completely different lab, who uses slides from Mercedes 
Medical (bad batch, maybe?),  who cut these sections for said professor. 
I keep telling him to either snitch a box of my slides to give to them, 
or let me cut for him-he himself has said that when I use my Gold Seal 
Ultrastick slides, those sections never fall off.  :o)

-Kathy

Peter Carroll wrote:

> next time, cut them onto plus slides ;)
>
> Kathleen Roberts wrote:
>
>> Hi all,
>>
>> I just had a professor come to me asking if I had any tricks to 
>> rescue a section that is partially off its slide.  This is 
>> formalin-fixed, paraffin embedded mouse brain, and one section on one 
>> slide is half attached and half floating after antigen retrieval for 
>> IHC (no, I don't know which retrieval method he used, but I can find 
>> out if you need it).  I've never tried to do this, and a search of 
>> the archives turned up nothing, but my keywords may be off.    Has 
>> anybody managed to save a section that was coming off the slide?
>>
>> Thanks,
>> Kathleen Roberts
>> Principal Lab Technician
>> Neurotoxicology Labs
>> Dept of Pharmacology & Toxicology
>> Ernest Mario School of Pharmacy
>> Rutgers University
>> 41 B Gordon Road
>> Piscataway, NJ 08854
>>
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>>
>