[Histonet] Re: need help with Elastase staining
Sarah Tarran
sarah_tarran <@t> wmi.usyd.edu.au
Tue Feb 10 20:55:23 CST 2009
Hi Patricia,
I use Dako's neutrophil elastase clone NP57 (code MO752) on FFPE tissue
sections. I couldn't get it to work until I did the peroxidase block after
the application of the primary antibody - I stain with the neutrophil
elastase for 1 hour, then apply H2O2 for 5 minutes (I use the ready to use
one from Dako), then I use Dako's EnVision as my detection system for 30
minutes. I hope this helps!
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> Today's Topics:
>
> 1. need help with Elastase staining (Patricia F Lott)
> 2. HPS stain (Gagnon, Eric)
> 3. Re: Disinfecting Cryostat (Caroline Miller)
> 4. AO 860 repairman in NC? (Caroline Bass)
> 5. Re: HPS stain (Robert Richmond)
> 6. special stain instrument (Santiago, Albert)
> 7. thermowave users (godsgalnow <@t> aol.com)
> 8. Techs from Rockford Health System (Steven Coakley)
> 9. Microtomy of LEEP specimens (Joanne Clark)
> 10. RE: Microtomy of LEEP specimens (Mike Pence)
> 11. Re: Microtomy of LEEP specimens (Victor Tobias)
> 12. RE: Microtomy of LEEP specimens (Laurie Colbert)
> 13. cotton blue=acid blue=methyl blue (Edwards, R.E.)
> 14. Breast tissue (rmweber113 <@t> comcast.net)
> 15. NY DOH inspection question (Whitaker, Bonnie)
> 16. Re: cotton blue=acid blue=methyl blue (Geoff McAuliffe)
> 17. RE: NY DOH inspection question (McMahon, Loralee A)
> 18. RE: Microtomy of LEEP specimens (Smith Wanda)
> 19. Re: Breast tissue (Rene J Buesa)
> 20. Ventana Ultra (thisisann <@t> aol.com)
> 21. spin columns (Emily Sours)
> 22. spin columns (Pat Flannery)
> 23. Re: Breast tissue (mari.ann.mailhiot <@t> leica-microsystems.com)
> 24. RE: RE: Microtomy of LEEP specimens (Tom McNemar)
> 25. Re: Breast tissue (Angela Bitting)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 9 Feb 2009 12:09:55 -0600
> From: "Patricia F Lott" <plott <@t> uab.edu>
> Subject: [Histonet] need help with Elastase staining
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <DCFAB2F06434294D8CF45C3E28C88D9E56C3B3 <@t> UABEXMB8.ad.uab.edu>
> Content-Type: text/plain; charset="US-ASCII"
>
> Does anyone have any experience with staining for Elastase in
> Neutrophils in tissue sections? Any help would be appreciated!
>
>
>
> ------------------------------
>
> Message: 2
> Date: Mon, 9 Feb 2009 13:11:50 -0500
> From: "Gagnon, Eric" <gagnone <@t> KGH.KARI.NET>
> Subject: [Histonet] HPS stain
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <F93BD6329FC3AE4C8DB116B985FBC31327C3A872 <@t> KGHMAIL.KGH.ON.CA>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi Sharon,
>
> HPS is our routine stain (instead of H&E, which 98% of labs use). Not
> sure what part of the stain you are having trouble with, but most
> unpredictability usually comes in the phloxine/saffron balance.
>
> Some things to watch for are:
> -keeping your saffron covered, and I would suggest parafilm-ing it when
> not in use
> -keep the alcohols before the saffron rigorously free of
> phloxine-contaminated alcohol, thus not allowing tainted alcohol to
> contaminate/dilute the saffron during staining
> -getting all the background hematoxylin and phloxine out, which can be
> checked as you go along when you validate your method
> -be diligent about the preparation of the saffron. In addition to
> Hermina's suggested method, we microwave the dry saffron, grind it, then
> boil the alcoholic mixture to remove as much moisture as possible
>
> What do you use the stain for, i.e. differentiating muscle and collagen?
> As a routine stain, I understand its use in Canada is limited to a few
> laboratories in the east such as the Ottawa, Kingston, Sudbury areas.
> This is often because pathologists who trained here at Queen's Pathology
> like to take the stain with them. Having said that, many of our residents
> request slides stained with H&E, to familiarize themselves with that stain
> before exams.
>
> Good luck, hope this helps...
>
> Eric Gagnon MLT
> Histology Laboratory,
> Kingston General Hospital
> Kingston, Ontario, Canada
>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Mon, 9 Feb 2009 10:17:19 -0800
> From: Caroline Miller <cmiller <@t> gladstone.ucsf.edu>
> Subject: Re: [Histonet] Disinfecting Cryostat
> To: lpwenk <@t> sbcglobal.net
> Cc: histonet <@t> lists.utsouthwestern.edu, 'Ingles Claire '
> <CIngles <@t> uwhealth.org>
> Message-ID: <407E13F3-8499-4B03-BB56-E7DC95A9448D <@t> gladstone.ucsf.edu>
> Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
>
> In the UK (where I used to run a clinical lab) we also would turn off
> the cryostat in the morning, wait for it to defrost then just before
> going home for the evening heat up some formalin in the microwave and
> place that in the chamber and leave overnight.
>
> It certainly kills everything, but it is also kinda toxic to your
> lungs! I wouldn't suggest it but it is a 'severe cleaning' option,
> especially if you have had something nasty in there.
>
> This was about 5 years ago, the rules may have changed, and Yes, I do
> realise just how bad that is for you and everyone around!
>
> Caroline
>
>
> Caroline Miller
> Co-Manager
> J David Gladstone Institutes Histology and Microscopy Core
> 1650 Owens St
> San Francisco
> CA 94158
>
> http://www.gladstone.ucsf.edu/gladstone/site/histology/
> cmiller <@t> gladstone.ucsf.edu
>
>
>
>
> On Feb 7, 2009, at 1:11 PM, Lee & Peggy Wenk wrote:
>
>> 95-100% also work, for bacteria, fungus, viruses. They will not work
>> on
>> killing spores. That's where the 70% is to be used, as the water
>> (30% part)
>> is needed to soften the spore wall, so that the alcohol (70% part)
>> can get
>> inside.
>>
>> Peggy A. Wenk, HTL(ASCP)SLS
>> Beaumont Hospital
>> Royal Oak, MI 48073
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ingles
>> Claire
>> Sent: Friday, February 06, 2009 6:38 PM
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: RE: [Histonet] Disinfecting Cryostat
>>
>> Why no 95%? We have been using it in perpituity with no bad effects.
>> The
>> alcohol has evaporated from the inside surfaces by morning.
>>
>> Claire
>>
>> ________________________________
>>
>> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of gayle
>> callis
>> Sent: Fri 2/6/2009 11:11 AM
>> To: 'Louise Hartson'; histonet <@t> lists.utsouthwestern.edu
>> Subject: RE: [Histonet] Disinfecting Cryostat
>>
>>
>>
>> We use 70% ethanol on gauze (just damp) to wipe down inside of
>> cryostat. Do
>> not use 95% or 100% alcohol, as 70% is the most effective for
>> disinfection,
>> commonly used in biohoods. We pick up little trimmings with first
>> dampened
>> gauze (or kim wipe) then re-wipe with a second gauze. We prefer
>> gauze for
>> its flexibility. Also, if you put a kimwipe or gauze behind knife
>> holder to
>> l catch trimmings then you can fold up kimwipe, and lift "garbage"
>> out,
>> intact, and go to biohazard container before wipe down. We try to
>> avoid too
>> much alcohol on knife holder parts since alcohol ruins lubricants
>> needed for
>> good operation. Check with your cryostat manufacturer to see what
>> they
>> recommend. It pays to wipe down under and over sliding glass door to
>> counterattack biohazardous "cling-ons" found on your gloves, and where
>> people have touched the instrument. 70% alcohol will not kill prions.
>>
>> Water based disinfectants freeze in cryostat. Eventually you need
>> scheduled
>> disinfection with cryostat in defrosted mode with approved
>> disinfecting
>> agent.
>>
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>> _______________________________________________
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>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Mon, 09 Feb 2009 13:47:31 -0500
> From: Caroline Bass <cbass <@t> wfubmc.edu>
> Subject: [Histonet] AO 860 repairman in NC?
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <C5B5E473.3410%cbass <@t> wfubmc.edu>
> Content-Type: text/plain; charset="ISO-8859-1"
>
> Hello Everyone,
>
> I have refurbished an old AO 860 sliding microtome. I¹m pretty proud of
> myself, I¹ve unlocked the totally gummed mechanism and have put it
> together
> myself. It seems to work very well. The only problem I have is tuning the
> sliding block. I have no idea how to approach it, and after several failed
> attempts (I can get it to slide down the length, but can¹t achieve a
> smooth
> movement), I¹m wondering if it¹s better to find a repairman in the area
> that
> can spend an hour showing me how to do this.
>
> Does anyone have suggestions? Do you know of any repairmen in the area?
>
> Thanks,
>
> Caroline Bass
>
>
> ------------------------------
>
> Message: 5
> Date: Mon, 9 Feb 2009 14:24:01 -0500
> From: Robert Richmond <RSRICHMOND <@t> aol.com>
> Subject: [Histonet] Re: HPS stain
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <abea52a60902091124h5d42780bg96bd654237f9cec9 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> The hematoxylin-phloxin- saffron stain, which supposedly originated at
> the Montreal Neurological Institute, migrated from there to
> Columbia-Presbyterian Hospital in New York City, where it was used as
> the general oversight stain in surgical pathology at least into the
> 1960's. When I saw it in use there in 1966 they didn't seem to be
> having trouble with it.
>
> On the other side of Central Park, New York Hospital (Cornell Medical
> Center) had used a Light Green trichrome stain as a general oversight
> stain. I think they abandoned it in favor of H & E after Chandler
> Foot's retirement in 1948.
>
> Saffron is a natural dye derived from the stigmas of the saffron
> crocus, Crocus sativus. Although it was (and is) extremely expensive,
> it was used both as a textile dye and as a spice (in paella, for
> example). It's sometimes seen (and smelled) as a component of
> expensive curry powders - my wife and I have been experimenting with
> Penzey's Maharajah curry powder, which is 2% saffron. The immense hand
> labor of extracting all those stigmas from the flowers makes saffron
> "the most expensive spice in the world".
>
> Saffron as a histologic dye has traditionally been specified as
> "safran du Gâtinais" - from a particular region of France - though I
> am not certain that such a product still actually exists. I would
> think that any good quality saffron would suffice (don't substitute
> safflower, 'dyer's saffron'). You'll pay at least ten dollars a gram
> for saffron, at Penzeys anyway.
>
> Some recipes specified as many as seven changes of hot alcohol to
> extract the dye, and some people used a reflux condenser for the
> extraction. The alcohol extract has a strong medicinal smell which
> some people find quite unpleasant.
>
> (I have no connection with Penzeys.com, except that my wife and I have
> gotten addicted to their shipments of very high quality spices.)
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
>
>
> ------------------------------
>
> Message: 6
> Date: Mon, 9 Feb 2009 14:57:24 -0500
> From: "Santiago, Albert" <Albert.Santiago <@t> uphs.upenn.edu>
> Subject: [Histonet] special stain instrument
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <BB0F4D2041EED9458AD95A538DBC4CAE02FA73D2 <@t> uphsmbx6.UPHS.PENNHEALTH.PRV>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello again fellow histonetters, I have an old artisan special stain/ihc
> stain stainer that I'm looking to replace with a newer model, but before
> I commit to it I was wondering if anyone else uses any other special
> stain instrument that I can look into and compare.... Thanks for your
> help....
>
>
>
> Albert Santiago, HT (ASCP)
>
> Laboratory Supervisor
>
> Dermatopathology
>
> Phone 215-662-6539
>
> Fax 215-662-6150
>
>
>
>
>
>
>
>
>
> The information contained in this e-mail message is intended only for the
> personal and confidential use of the recipient(s) named above. If the
> reader of this message is not the intended recipient or an agent
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>
> ------------------------------
>
> Message: 7
> Date: Mon, 09 Feb 2009 15:03:47 -0500
> From: godsgalnow <@t> aol.com
> Subject: [Histonet] thermowave users
> To: histonet <@t> pathology.swmed.edu
> Message-ID: <8CB5908A749D8F9-F58-E84 <@t> webmail-da13.sysops.aol.com>
> Content-Type: text/plain; charset="us-ascii"
>
> Can any ThermoWave users that microwave prostates please email me offline,
> if you are willing to share SOPs....
>
>
> Thanks,
>
> Roxanne
>
>
> ------------------------------
>
> Message: 8
> Date: Mon, 9 Feb 2009 12:04:49 -0800 (PST)
> From: Steven Coakley <sjchtascp <@t> yahoo.com>
> Subject: [Histonet] Techs from Rockford Health System
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <219757.8126.qm <@t> web38205.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> Wondering if there are any tech here from Rockford Health System,
> Rockford, Ill.
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Mon, 9 Feb 2009 13:39:11 -0700
> From: "Joanne Clark" <jclark <@t> pcnm.com>
> Subject: [Histonet] Microtomy of LEEP specimens
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39CF66519 <@t> mail.pcnm.com>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi,
>
>
>
> How many of you have a protocol to cut multiple deepers/levels on LEEP
> specimens and if so, how many do you cut?
>
>
>
> Thanks
>
> Joanne Clark, HT, MLT
>
> Pathology Consultants of New Mexico
>
> Roswell, NM
>
>
>
> ------------------------------
>
> Message: 10
> Date: Mon, 9 Feb 2009 14:59:33 -0600
> From: "Mike Pence" <mpence <@t> grhs.net>
> Subject: RE: [Histonet] Microtomy of LEEP specimens
> To: "Joanne Clark" <jclark <@t> pcnm.com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <661949901A768E4F9CC16D8AF8F2838C017A3A83 <@t> IS-E2K3.grhs.net>
> Content-Type: text/plain; charset="us-ascii"
>
> We cut 3 levels on each block.
> Mike
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joanne
> Clark
> Sent: Monday, February 09, 2009 2:39 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Microtomy of LEEP specimens
>
>
> Hi,
>
>
>
> How many of you have a protocol to cut multiple deepers/levels on LEEP
> specimens and if so, how many do you cut?
>
>
>
> Thanks
>
> Joanne Clark, HT, MLT
>
> Pathology Consultants of New Mexico
>
> Roswell, NM
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Mon, 09 Feb 2009 14:08:14 -0800
> From: Victor Tobias <victor <@t> pathology.washington.edu>
> Subject: Re: [Histonet] Microtomy of LEEP specimens
> To: Joanne Clark <jclark <@t> pcnm.com>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <4990A94E.9030001 <@t> pathology.washington.edu>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> 3 levels.
>
> Victor Tobias
> Clinical Applications Analyst
> University of Washington Medical Center
> Dept of Pathology Room BB220
> 1959 NE Pacific
> Seattle, WA 98195
> victor <@t> pathology.washington.edu
> 206-598-2792
> 206-598-7659 Fax
> =================================================
> Privileged, confidential or patient identifiable information may be
> contained in this message. This information is meant only for the use
> of the intended recipients. If you are not the intended recipient, or
> if the message has been addressed to you in error, do not read,
> disclose, reproduce, distribute, disseminate or otherwise use this
> transmission. Instead, please notify the sender by reply e-mail, and
> then destroy all copies of the message and any attachments.
>
>
>
> Joanne Clark wrote:
>> Hi,
>>
>>
>>
>> How many of you have a protocol to cut multiple deepers/levels on LEEP
>> specimens and if so, how many do you cut?
>>
>>
>>
>> Thanks
>>
>> Joanne Clark, HT, MLT
>>
>> Pathology Consultants of New Mexico
>>
>> Roswell, NM
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>
> ------------------------------
>
> Message: 12
> Date: Mon, 9 Feb 2009 14:10:46 -0800
> From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
> Subject: RE: [Histonet] Microtomy of LEEP specimens
> To: "Joanne Clark" <jclark <@t> pcnm.com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <57BE698966D5C54EAE8612E8941D768304E9D603 <@t> EXCHANGE3.huntingtonhospital.com>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Three levels on all blocks
>
> Laurie Colbert
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joanne
> Clark
> Sent: Monday, February 09, 2009 12:39 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Microtomy of LEEP specimens
>
> Hi,
>
>
>
> How many of you have a protocol to cut multiple deepers/levels on LEEP
> specimens and if so, how many do you cut?
>
>
>
> Thanks
>
> Joanne Clark, HT, MLT
>
> Pathology Consultants of New Mexico
>
> Roswell, NM
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 13
> Date: Tue, 10 Feb 2009 11:45:11 +0000
> From: "Edwards, R.E." <ree3 <@t> leicester.ac.uk>
> Subject: [Histonet] cotton blue=acid blue=methyl blue
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <7722595275A4DD4FA225B92CDBF174A1745423DC64 <@t> EXC-MBX3.cfs.le.ac.uk>
> Content-Type: text/plain; charset="us-ascii"
>
>
> Looking for a supplier please, I am aware that Sigma/Fluka supply a
> ready made solution, which apparently does not work.
>
> Many thanks
>
> Richard Edwards
> Leicester University
>
> U.K.
>
>
>
>
>
> ------------------------------
>
> Message: 14
> Date: Tue, 10 Feb 2009 13:49:58 +0000 (UTC)
> From: rmweber113 <@t> comcast.net
> Subject: [Histonet] Breast tissue
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <1347926049.554711234273798846.JavaMail.root <@t> sz0046a.westchester.pa.mail.comcast.net>
>
> Content-Type: text/plain; charset=utf-8
>
>
>
>
> Hello,   Do anyone have a better remedy to process fatty breast tissue
> other than after processing pressing it in paper towels and then putting
> it back in paraffin for a couple of hours.  The pathologist are saying
> this is taking to long.
>
>
>
> Thanks,
>
>
>
>
>
>
> ------------------------------
>
> Message: 15
> Date: Tue, 10 Feb 2009 09:26:02 -0500
> From: "Whitaker, Bonnie" <Bonnie.Whitaker <@t> osumc.edu>
> Subject: [Histonet] NY DOH inspection question
> To: HistoNet <@t> pathology.swmed.edu
> Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F60801E52 <@t> msxc06.OSUMC.EDU>
> Content-Type: text/plain; charset=us-ascii
>
> Hi All,
>
> Will some of you that are inspected by the NYDOH please email me with how
> you
> meet the requirement (below) for performance verification for alcohol,
> xylene
> and formalin?
> Clinical Laboratory Standards of Practice, Part 1- General Systems;
> Reagents
> Sustaining Standard of Practice 4 (REAG S4): Inventory Control.
> The standard states that the inventory log should include the following
> information: lot numbers, expiration dates, the date of receipt in the
> laboratory, date of performance verification and the date the material is
> placed in service, it is a requirement.
>
>
> Thanks!!
>
> Bonnie Whitaker
> Clinical Histology Manager
> Ohio State University Medical Center
> 614.293.5048
>
>
>
> ------------------------------
>
> Message: 16
> Date: Tue, 10 Feb 2009 09:55:10 -0500
> From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
> Subject: Re: [Histonet] cotton blue=acid blue=methyl blue
> To: "Edwards, R.E." <ree3 <@t> leicester.ac.uk>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <4991954E.7020401 <@t> umdnj.edu>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> Sounds like you want Aniline Blue, CI 42780. Much easier to find by that
> name.
>
> Geoff
>
> Edwards, R.E. wrote:
>> Looking for a supplier please, I am aware that Sigma/Fluka supply
>> a ready made solution, which apparently does not work.
>>
>> Many thanks
>>
>> Richard Edwards
>> Leicester University
>>
>> U.K.
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
>
>
> --
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583
> mcauliff <@t> umdnj.edu
> **********************************************
>
>
>
>
>
> ------------------------------
>
> Message: 17
> Date: Tue, 10 Feb 2009 09:58:43 -0500
> From: "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu>
> Subject: RE: [Histonet] NY DOH inspection question
> To: "Whitaker, Bonnie" <Bonnie.Whitaker <@t> osumc.edu>,
> <HistoNet <@t> pathology.swmed.edu>
> Message-ID:
> <2CF6F6B05263EA4EBAB07781B51E5DB002C945D8 <@t> e2k3ms1.urmc-sh.rochester.edu>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I would be interested in how you verify the performance of the xylenes and
> alcohols as well. Thank you in advance.
>
> Loralee McMahon, HTL (ASCP)
> ICC Supervisor
> University of Rochester
> Department of Pathology
>
> (585) 275-7210
>
>
> ________________________________
>
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Whitaker,
> Bonnie
> Sent: Tue 2/10/2009 9:26 AM
> To: HistoNet <@t> pathology.swmed.edu
> Subject: [Histonet] NY DOH inspection question
>
>
>
> Hi All,
>
> Will some of you that are inspected by the NYDOH please email me with how
> you
> meet the requirement (below) for performance verification for alcohol,
> xylene
> and formalin?
> Clinical Laboratory Standards of Practice, Part 1- General Systems;
> Reagents
> Sustaining Standard of Practice 4 (REAG S4): Inventory Control.
> The standard states that the inventory log should include the following
> information: lot numbers, expiration dates, the date of receipt in the
> laboratory, date of performance verification and the date the material is
> placed in service, it is a requirement.
>
>
> Thanks!!
>
> Bonnie Whitaker
> Clinical Histology Manager
> Ohio State University Medical Center
> 614.293.5048
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 18
> Date: Tue, 10 Feb 2009 09:06:02 -0600
> From: Smith Wanda <Wanda.Smith <@t> HCAhealthcare.com>
> Subject: [Histonet] RE: Microtomy of LEEP specimens
> To: Joanne Clark <jclark <@t> pcnm.com>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <9E2D36CE2D7CBA4A94D9B22E8328A3BACBE95606 <@t> NADCWPMSGCMS03.hca.corpad.net>
>
> Content-Type: text/plain; charset="us-ascii"
>
> We routinely cut 3 levels on 3 slides for each block.
>
>
> WANDA G. SMITH, HTL(ASCP)HT
> Pathology Supervisor
> TRIDENT MEDICAL CENTER
> 9330 Medical Plaza Drive
> Charleston, SC 29406
> 843-847-4586
> 843-847-4296 fax
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joanne
> Clark
> Sent: Monday, February 09, 2009 3:39 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Microtomy of LEEP specimens
>
> Hi,
>
>
>
> How many of you have a protocol to cut multiple deepers/levels on LEEP
> specimens and if so, how many do you cut?
>
>
>
> Thanks
>
> Joanne Clark, HT, MLT
>
> Pathology Consultants of New Mexico
>
> Roswell, NM
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 19
> Date: Tue, 10 Feb 2009 07:21:09 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Breast tissue
> To: histonet <@t> lists.utsouthwestern.edu, rmweber113 <@t> comcast.net
> Message-ID: <636989.62353.qm <@t> web65701.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> The "better remedy" is to cut the slices thin and fix/process correctly,
> specially using mineral oil.
> René J.
>
> --- On Tue, 2/10/09, rmweber113 <@t> comcast.net <rmweber113 <@t> comcast.net>
> wrote:
>
> From: rmweber113 <@t> comcast.net <rmweber113 <@t> comcast.net>
> Subject: [Histonet] Breast tissue
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Tuesday, February 10, 2009, 8:49 AM
>
>
> Hello, Do anyone have a better remedy to process fatty breast tissue
> other than after processing pressing it in paper towels and then putting
> it back
> in paraffin for a couple of hours. The pathologist are saying this is
> taking
> to long.
>
>
>
> Thanks,
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 20
> Date: Tue, 10 Feb 2009 10:34:13 -0500
> From: thisisann <@t> aol.com
> Subject: [Histonet] Ventana Ultra
> To: histonet <@t> lists.utsouthwestern.edu
> Cc: ali.malik <@t> ventana.roche.com
> Message-ID: <8CB59AC28EAADEE-708-DC <@t> mblk-d24.sysops.aol.com>
> Content-Type: text/plain; charset="us-ascii"
>
> I am considering purchasing a Ventana Benchmark Ultra IHC.? Does anyone
> have any feedback concerning this piece of equipment.? Any feedback would
> be appreciated.
> Thanks,
> Ann
>
>
> ------------------------------
>
> Message: 21
> Date: Tue, 10 Feb 2009 10:36:56 -0500
> From: Emily Sours <talulahgosh <@t> gmail.com>
> Subject: [Histonet] spin columns
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <b39794b0902100736k767f8bb6v827cff813efdddfc <@t> mail.gmail.com>
> Content-Type: text/plain; charset=UTF-8
>
> Hello
>
> Anyone know where to purchase QIAquick spin columns? If you get them from
> Qiagen, they aren't sold separately, you have to buy them in a complete
> kit. I have a feeling I'm stuck doing so.
>
> Emily
> --
> It's like hearing Billy Joel play "Piano Man"-- joyless for all involved,
> but demanded by a higher power.
> --Kevin Murphy, Indiana Jones and the Crystal Skull rifftrax
>
>
> ------------------------------
>
> Message: 22
> Date: Tue, 10 Feb 2009 10:58:22 -0500
> From: Pat Flannery <pjfnefro <@t> duke.edu>
> Subject: [Histonet] spin columns
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <64D46715-499C-48B9-8CFA-EDBEBF95B810 <@t> duke.edu>
> Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
>
> Emily-
>
> I just got a mailing from Genesee Scientific (800-789-5550, Ext. 2)
> for their UniPrep columns. They're selling them specially for those
> people who always run out of columns before they run out of reagents.
> I think there are a couple of types, so you might want to contact them
> to see which one you'd need.
>
> Hope this helps.
>
> -Pat Flannery
> Duke Med Center
>
>
>> Hello
>>
>> Anyone know where to purchase QIAquick spin columns? If you get them
>> from
>> Qiagen, they aren't sold separately, you have to buy them in a
>> complete
>> kit. I have a feeling I'm stuck doing so.
>>
>> Emily
>> --
>> It's like hearing Billy Joel play "Piano Man"-- joyless for all
>> involved,
>> but demanded by a higher power.
>> --Kevin Murphy, Indiana Jones and the Crystal Skull rifftrax
>> _______________________________________________
>
>
>
> ------------------------------
>
> Message: 23
> Date: Tue, 10 Feb 2009 10:10:53 -0600
> From: mari.ann.mailhiot <@t> leica-microsystems.com
> Subject: Re: [Histonet] Breast tissue
> To: rmweber113 <@t> comcast.net
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <OFFD3C1DAD.63E32CCA-ON86257559.0057B91E-86257559.0059237B <@t> leica-microsystems.com>
>
> Content-Type: text/plain; charset=ISO-8859-1
>
> As long as you smell the clearant in the block then putting the blocks in
> paraffin longer works. I never found it necessary to roll the fat in paper
> towel and place the specimen back in paraffin. If the block is not fixed
> ore dehydrated properly and cleared properly putting them in paraffin
> doesn't work.
>
> It all starts at the gross station. Cutting nice thin sections that are
> fixed for at least 24 hrs. Even before that breast should be bread loafed
> and placed in formalin and fixed for 24 hrs. Then a section is cut and
> placed in a cassette and that sits in formalin until it gets placed on the
> processor.
>
> The process of rolling fatty tissue in paper towel and gently pressed
> allows the fatty specimen to go back in formalin and be reprocessed
> without
> taking the specimen back through the xylenes and alcohols to reprocess.
>
> Hope this helps.
>
> Best regards
>
> Mari Ann Mailhiot BA HT ASCP
> Application Specialist/Trainer
> Leica Microsystems
> Biosystems Division
> Technical Assistance Center
> 800 248 0123 x7267
> 847 236 3063 fax
> mari.ann.mailhiot <@t> leica-microsystems.com
> www.leica-microsystems.com
>
>
>
> rmweber113 <@t> comcas
> t.net
> Sent by: To
> histonet-bounces@ histonet <@t> lists.utsouthwestern.edu
> lists.utsouthwest cc
> ern.edu
> Subject
> [Histonet] Breast tissue
> 02/10/2009 07:49
> AM
>
>
>
>
>
>
>
>
>
>
> Hello, Do anyone have a better remedy to process fatty breast tissue
> other than after processing pressing it in paper towels and then putting
> it
> back in paraffin for a couple of hours. The pathologist are saying this
> is taking to long.
>
>
>
> Thanks,
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ______________________________________________________________________
> This email has been scanned by the MessageLabs Email Security System.
> For more information please visit http://www.messagelabs.com/email
> ______________________________________________________________________
>
>
>
> ------------------------------
>
> Message: 24
> Date: Tue, 10 Feb 2009 11:36:31 -0500
> From: "Tom McNemar" <TMcNemar <@t> lmhealth.org>
> Subject: RE: [Histonet] RE: Microtomy of LEEP specimens
> To: "Smith Wanda" <Wanda.Smith <@t> HCAhealthcare.com>, "Joanne Clark"
> <jclark <@t> pcnm.com>, <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E0A2 <@t> lmhsmail.lmhealth.org>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Same here.
>
> Tom McNemar, HT(ASCP)
> Histology Co-ordinator
> Licking Memorial Health Systems
> (740) 348-4163
> (740) 348-4166
> tmcnemar <@t> lmhealth.org
> www.LMHealth.org
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Smith
> Wanda
> Sent: Tuesday, February 10, 2009 10:06 AM
> To: Joanne Clark; histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] RE: Microtomy of LEEP specimens
>
>
> We routinely cut 3 levels on 3 slides for each block.
>
>
> WANDA G. SMITH, HTL(ASCP)HT
> Pathology Supervisor
> TRIDENT MEDICAL CENTER
> 9330 Medical Plaza Drive
> Charleston, SC 29406
> 843-847-4586
> 843-847-4296 fax
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joanne
> Clark
> Sent: Monday, February 09, 2009 3:39 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Microtomy of LEEP specimens
>
> Hi,
>
>
>
> How many of you have a protocol to cut multiple deepers/levels on LEEP
> specimens and if so, how many do you cut?
>
>
>
> Thanks
>
> Joanne Clark, HT, MLT
>
> Pathology Consultants of New Mexico
>
> Roswell, NM
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 25
> Date: Tue, 10 Feb 2009 12:09:45 -0500
> From: "Angela Bitting" <akbitting <@t> geisinger.edu>
> Subject: Re: [Histonet] Breast tissue
> To: <rmweber113 <@t> comcast.net>,
> <mari.ann.mailhiot <@t> leica-microsystems.com>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <49916E89.2B7F.00C9.0 <@t> geisinger.edu>
> Content-Type: text/plain; charset=US-ASCII
>
> We extended our paraffin infiltration times and that worked out well for
> us too.
>
>>>> <mari.ann.mailhiot <@t> leica-microsystems.com> 2/10/2009 11:10 AM >>>
> As long as you smell the clearant in the block then putting the blocks in
> paraffin longer works. I never found it necessary to roll the fat in paper
> towel and place the specimen back in paraffin. If the block is not fixed
> ore dehydrated properly and cleared properly putting them in paraffin
> doesn't work.
>
> It all starts at the gross station. Cutting nice thin sections that are
> fixed for at least 24 hrs. Even before that breast should be bread loafed
> and placed in formalin and fixed for 24 hrs. Then a section is cut and
> placed in a cassette and that sits in formalin until it gets placed on the
> processor.
>
> The process of rolling fatty tissue in paper towel and gently pressed
> allows the fatty specimen to go back in formalin and be reprocessed
> without
> taking the specimen back through the xylenes and alcohols to reprocess.
>
> Hope this helps.
>
> Best regards
>
> Mari Ann Mailhiot BA HT ASCP
> Application Specialist/Trainer
> Leica Microsystems
> Biosystems Division
> Technical Assistance Center
> 800 248 0123 x7267
> 847 236 3063 fax
> mari.ann.mailhiot <@t> leica-microsystems.com
> www.leica-microsystems.com
>
>
>
> rmweber113 <@t> comcas
> t.net
> Sent by: To
> histonet-bounces@ histonet <@t> lists.utsouthwestern.edu
> lists.utsouthwest cc
> ern.edu
> Subject
> [Histonet] Breast tissue
> 02/10/2009 07:49
> AM
>
>
>
>
>
>
>
>
>
>
> Hello, Do anyone have a better remedy to process fatty breast tissue
> other than after processing pressing it in paper towels and then putting
> it
> back in paraffin for a couple of hours. The pathologist are saying this
> is taking to long.
>
>
>
> Thanks,
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ______________________________________________________________________
> This email has been scanned by the MessageLabs Email Security System.
> For more information please visit http://www.messagelabs.com/email
> ______________________________________________________________________
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> IMPORTANT WARNING: The information in this message (and the documents
> attached to it, if any) is confidential and may be legally privileged. It
> is intended solely for the addressee. Access to this message by anyone
> else is unauthorized. If you are not the intended recipient, any
> disclosure, copying, distribution or any action taken, or omitted to be
> taken, in reliance on it is prohibited and may be unlawful. If you have
> received this message in error, please delete all electronic copies of
> this message (and the documents attached to it, if any), destroy any hard
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>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 63, Issue 12
> ****************************************
>
Sarah Tarran
Postdoctoral Fellow
Vascular Biology Research Centre,
Department of Surgery
Westmead Hospital, Westmead, NSW, 2145
02 98455775 or 0408119276
sarah_tarran <@t> wmi.usyd.edu.au
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