[Histonet] Mountant for GMA stained sections

Pamela Marcum pmarcum <@t> vet.upenn.edu
Fri Feb 6 09:08:48 CST 2009


I agree with Gayle.  We put a weight on the coverslip until it dries to help
keep it flat.  Since all mounting media has some organic solvent like xylene
or toluene it is hard to avoid them completely.  We use DPX for everything
here.

Best Regards,

Pamela A Marcum
University of Pennsylvania 
School of Veterinary Medicine
Comparative Orthopedic Laboratory (CORL)
382 W Street Rd
Kennett Square PA 19438
610-925-6278

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of gayle callis
Sent: Friday, February 06, 2009 9:46 AM
To: 'Histonet'; ' Histonet messages replies'
Subject: [Histonet] Mountant for GMA stained sections

Let stained GMA sections  air dry and mount with a permanent mounting media.
Dipping in xylene tends to crack or cause the GMA to wrinkle since the
plastic can't be removed.  

 

Gayle M. Callis 

HTL(ASCP)HT,MT

Bozeman MT

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