[Histonet] Does anybody use PARP?

[Jim Manavis]

Alexandra

I have used it and it's a very good antibody. It works extremely well in
both Human and rat tissue. We use at a dilution of 1/2000 (overnight
incubation) with EDTA antigen retrieval using a streptavidin detection
system.

Cheers

Jim

Jim Manavis
Laboratory Manager
Hanson Institute
Centre for Neurological Diseases
IMVS, Adelaide, SA, 5000
Australia
Phone: 61-08-8222-3668 / 0401120697
FAX: 61-08-8222 3392
email: jim.manavis <@t> imvs.sa.gov.au
Disclaimer: Not this little black duck! 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Alexandra
Meinl
Sent: Wednesday, 4 February 2009 9:52 PM
To: Histonet
Subject: [Histonet] Does anybody use PARP?

Hello,

Does anybody use Promega's Anti-PARP p85 Fragment on rat FFPE tissue
sucessfully?
Do you have good results or would you recommend another antibody?

Alexandra
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