[Histonet] PFA/sucrose cryopreservation of endogenous YFP signal

Jo Dee Fish jfish <@t> gladstone.ucsf.edu
Tue Feb 3 13:47:30 CST 2009

Hi Scott,
"slowly freezing on dry ice" may be your problem.  In my honest opinion, you
need to freeze faster.  Try a different method, such as cooled isopentane,
either with LN2 (coolest method) or dry ice.  You should get better results
when you freeze them faster or colder.
Good luck,
Jo Dee

~~Jo Dee Fish~~
Senior Research Technologist
The J. David Gladstone Institutes
Co-manager Histology and Microscopy Core
Telephone: (415) 734-2567
Fax: (415) 355-0824
E-mail: jfish <@t> gladstone.ucsf.edu
Mailing address:
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA 94158

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