[Histonet] PFA/sucrose cryopreservation of endogenous YFP signal

[Jo Dee Fish]

Hi Scott,
"slowly freezing on dry ice" may be your problem.  In my honest opinion, you
need to freeze faster.  Try a different method, such as cooled isopentane,
either with LN2 (coolest method) or dry ice.  You should get better results
when you freeze them faster or colder.
Good luck,
Jo Dee


~~Jo Dee Fish~~
Senior Research Technologist
The J. David Gladstone Institutes
Co-manager Histology and Microscopy Core
 
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