[Histonet] reprocessing

mari.ann.mailhiot <@t> leica-microsystems.com mari.ann.mailhiot <@t> leica-microsystems.com
Tue Feb 3 13:32:15 CST 2009


I remember reading an article maybe in the Histology Journal awhile ago. In
there it recommended rolling and blotting the tissue with a paper towel to
remove excess paraffin and then placing the specimen in a cassette and
putting that cassette directly into formalin. I never had an issues with
this process.

Sometimes I checked the block to see if I smell xylene. If I do smell
xylene that usually means the block needs more time in paraffin. We then
usually dropped the blocks in the paraffin tank on the embedding center.
They usually stayed there a few hours. Never was any of the fatty tissue
compromised from the extended time in paraffin.

All of this being said I worked at a very busy placed and we replaced
paraffin in our tanks everyday. So paraffin was always clean and never
affected by a few blacks place in the embedding tank.

Just some thoughts.

Best Regards

Mari Ann Mailhiot BA HT ASCP
Application Specialist/Trainer
Leica Microsystems
Biosystems Division
Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot <@t> leica-microsystems.com

             kristen arvidson                                              
             yahoo.com>                                                 To 
             Sent by:                  histonet                            
             histonet-bounces@         <histonet <@t> lists.utsouthwestern.edu> 
             lists.utsouthwest                                          cc 
                                       [Histonet] reprocessing             
             02/03/2009 12:13                                              
             Please respond to                                             
             arvidsonkristen <@t> y                                             

What are people doing these days when they have to reprocess fatty tissue?

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email 

More information about the Histonet mailing list