[Histonet] Histo gel Issues

Esther Peters esther.peters <@t> verizon.net
Mon Feb 2 16:58:47 CST 2009

We've had this happen with agarose, too, quite inconsistent results.  
Two pieces in same cassette, one fine, other shrunken.  Would love to 
know how to avoid the drying and hardening!

Esther Peters, Ph.D.
George Mason University

pam plumlee wrote:
> Dear Group: I'm having problems with the processing of
> small tissues in histogel. I follow the suggested
> embedding directions on the package and then process
> the gel blocks.  The results are very
> inconsistent-ideally, I'll get nice soft gel
> blocks-but, usually in a batch of 10 I get 2 good
> blocks and 8 dried up, flat hard squares.  They are
> all handled and processed the same.  Anyone experience
> this before?  Thanks for any input.
> Pam Plumlee H.T.
> Pfizer La Jolla
> pam.plumlee <@t> pfizer.com
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