[Histonet] IHC and ISH

Greg Dobbin gvdobbin <@t> ihis.org
Mon Feb 2 13:28:28 CST 2009


Jean,
The Bond Max is different than its main competitor in that the Bond
uses the same detection kit for both IHC and ISH. One just has to buy
the fluorocein reagent for the ISH method. So all you have is one
reagent to worry about expiring rather than an entire detection system.
Other instruments (they know who they are) require you to buy an entire
separate detection system to do ISH and so if your volumes for ISH are
low, you will waste a lot of money in expired kits. 
Cheers.
Greg 

Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

"I find that the harder I work, the 
more luck I seem to have."
- Thomas Jefferson


>>> "Taylor, Jean" <jtaylor <@t> meriter.com> 2/2/2009 1:50:41 PM >>>
Hi everyone,

I am currently in the process of evaluating IHC equipment to replace
my
old Dako Autostainers. The pathologists I work for are interested in
bringing ISH into our lab. My questions are, what IHC instrument have
you found works the best for IHC and ISH, and what kind of training
did
your personnel go through to become qualified to perform ISH?

Thank you!

Jean Taylor, HT(ASCP)QIHC
ICH Tech
Meriter Labs
Madison, WI 

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