[Histonet] RE: Histonet Digest, Vol 73, Issue 39,
Question 10. Dermlab
Valantou Grover
vgrover <@t> polysciences.com
Wed Dec 30 13:28:20 CST 2009
Rebecca,
There are many companies that will set up the lab from beginning to end,
TBS, Inc. has this service. You can contact them at 919-384-9393.
Valantou Grover, HT/HTL(ASCP), PA, MBA
Biosciences Product Line Manager
Polysciences, Inc.
400 Valley Road
Warrington, PA 18976
Fax: 1-800-343-3291
Phone number: 1-800-523-2575 X7418
Direct:1-215-488-7418
Cell phone: 1-215-409-8327
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Subject: Histonet Digest, Vol 73, Issue 39
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Today's Topics:
1. Auto Reply (Baustin)
2. Part Time MOHS Tech Needed in Portland (Eric Weber)
3. Re: Recycling Formalin, Xylene, and Alcohol (rgrow <@t> bmnet.com)
4. 2x3 microtomy: Adapter vs Sliding Microtome (R C)
5. Re: Stain to differentiate Hemoglobin from Hemosiderin?
(Robert Richmond)
6. LEICA 2055 AUTOCUT (Lucy Zong)
7. Re: Stain to differentiate Hemoglobin from Hemosiderin?
(John Kiernan)
8. Probe for mouse Y chromosome for FISH
(birnbaumm <@t> asaf.health.gov.il)
9. FNA code (Demarinis, Carolyn)
10. Derm Lab (Eric Sulkosky)
11. Re: FNA code (DKBoyd <@t> chs.net)
12. Re: Probe for mouse Y chromosome for FISH (Xipamanine Mkuze)
13. RE: LEICA 2055 AUTOCUT (Feher, Stephen)
14. Mississippi Histotechnology Homecoming (Jerry Santiago)
----------------------------------------------------------------------
Message: 1
Date: Tue, 29 Dec 2009 13:06:33 -0500
From: Baustin <baustin <@t> cbgbiotech.com>
Subject: [Histonet] Auto Reply
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1000171728 <@t> mail-server.cbgbiotech.com>
Content-Type: text/plain; charset="utf-8"
Please do not respond to this automatic e-mail reply.
I will be out of the office until Monday, January 4, 2010. If you need to
place an order, please email it to supplies <@t> cbgbiotech.com or fax it to
614-863-1676 Attn Ordering.
If you need to place a credit card order, please call 1-800-941-9484 and
dial ext 201 or 225.
Please note that all orders have been confirmed. If you did not receive a
fax confirmation, your order was not received by CBG.
------------------------------
Message: 2
Date: Tue, 29 Dec 2009 14:53:37 -0500
From: Eric Weber <erweber <@t> maxhealth.com>
Subject: [Histonet] Part Time MOHS Tech Needed in Portland
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9D12D4EF30176D4F839EB47F3D0E843611FAAF83 <@t> exbk2.maxhealth.com>
Content-Type: text/plain; charset="us-ascii"
MOHs Tech needed at the Portland VAMC for a Part Time Basis. Please
contact me if you would be interested. Details:
Dermatology MOHS Health Technician
The Portland VA Medical Center is in need of one part-time Dermatology
MOHS technician for the Dermatology Clinic, Operative Care Program at
the Portland VA Medical Center, 3710 SW US Veterans Hospital Road,
Portland, OR 97239.
Qualifications: Knowledge of basic methods and procedures of the
Dermatology clinic. Candidate shall demonstrate skill and precision in
use of the tools, materials and equipment for Dermatology clinic.
Knowledge of specialized terminology used in the specialized area of the
facility where the work is performed. Strong customer service skills;
detail oriented; and adaptability to work in a fast paced work
environment. Candidate will serve as technical expert in the operation
and maintenance of specialized equipment and instruments.
Experience: Basic computer knowledge. Candidate shall demonstrate
experience and skill in cutting and preparing frozen sections of tissue
in a Dermatology MOHS clinic. Familiarity with medical terminology and
familiarity with routine laboratory values and abbreviations are
required.
Specialty Experience (a Plus but not required): Familiarity with the VA
electronic medical record system (CPRS).
Length of Assignment: One year, or longer.
On-Call Hours: Not available
Overtime: Not available
Tour of Duty: Mondays for 8 hours, (7:30am to 3:30pm), Fridays for 5
hours (7:30 am to 12:30 pm).
Eric Weber
Maxim Government Services
7227 Lee DeForest Dr
Columbia, MD 21046
phone: (410) 910-4942
toll free: (866) 260-9142
fax:(410) 953-8358
erweber <@t> maxhealth.com
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------------------------------
Message: 3
Date: Tue, 29 Dec 2009 16:04:22 -0500
From: rgrow <@t> bmnet.com
Subject: [Histonet] Re: Recycling Formalin, Xylene, and Alcohol
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OFA3E10728.1D58BC58-ON8525769B.007307A4-8525769B.0073C473 <@t> bmnet.com>
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Andria,
CBG is very reliable. I have one of the large capacity units called: Solv
Solv. It's set up to run xylene on one side and alcohol on the other.
Very convenient, no flushes to do. I don't recommend recycling formalin,
to much fume exposure, testing, rebuffering, etc., but this unit will work
for formalin too.
Renee Grow, BA., HT (ASCP)
rgrow <@t> bmnet.com
Histology Supervisor
Blount Memorial Hospital
907 E. Lamar Alexander Pkwy.
Maryville, TN 37804-5016
(865) 977-4744
(865) 977-5766 Fax
You wrote:
Message: 4
Date: Mon, 21 Dec 2009 14:19:36 -0500
From: "Evans, Andria B" <aevans <@t> wellspan.org>
Subject: [Histonet] Recycling Formalin, Xylene, and Alcohol
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<A3E1DFCF072D6046AABF519AE828ADB107BF6962 <@t> EXCH4.wellspan.org>
Content-Type: text/plain; charset="iso-8859-1"
I would like to know what everyone out in histoland is using to recycle
there solutions. We are looking at switching out the procyclers (that are
about 10 years old) to something else. Any Pros/Cons of current methods
would be great. Thanks!!
Andria B Evans, HTL(ASCP)CM
Anatomic Pathology
York Hospital
1001 S. George Street
York, PA 17405
717-851-5006
------------------------------
Message: 4
Date: Tue, 29 Dec 2009 14:16:04 -0800
From: R C <ruebenjcarter <@t> gmail.com>
Subject: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<2a926e3f0912291416u723bd5d4ya6b93e199b4a3d0 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi. I'm working on a protocol for cutting monkey brain sections to be
mounted on 2x3 slides. I've read about utilizing a sliding microtome but in
short, have decided to use the 2x3 adapter for a standard Microm microtome.
During microtomy I've noticed many wrinkles in the sections, particularly
within the folds of the cerebellum. The wrinkles worsen as the sections
float in the water bath (temp=38).
In troubleshooting, a co-worker suggests inadequate fixation. I on the other
hand believe that the wrinkles relate to the Type R paraffin, which contains
polymers as well as the use of the adapter versus the sliding microtome.
Can anyone offer any first hand experience/guidance?
Thanks
------------------------------
Message: 5
Date: Tue, 29 Dec 2009 17:26:22 -0500
From: Robert Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Stain to differentiate Hemoglobin from
Hemosiderin?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<abea52a60912291426y68ea4080h94c2f627141a1335 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Jerry Ricks, Research Scientist, University of Washington,Department
of Pathology asks:
>>I've seen Prussian blue as a stain for hemosiderin but that would also
stain hemoglobin.<<
The Perls prussian blue reaction occurs with hemosiderin ("stainable
iron") but not with hemoglobin, hematin, formalin pigment, malarial
pigment, or melanin.
It isn't a stain, but a reaction between ferric iron and ferrocyanide
ion that produces a dense blue precipitate.
The Stainsfile page doesn't really answer your questions, and I'd
suggest looking it up in some of the standard textbooks.
Bob Richmond
Samurai Pathologist
Knoxville TN
------------------------------
Message: 6
Date: Tue, 29 Dec 2009 18:05:10 -0500
From: Lucy Zong <lucy.zong <@t> gmail.com>
Subject: [Histonet] LEICA 2055 AUTOCUT
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<8daef62e0912291505l7721b8acw2c339e97a29ddb6f <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
I was given a Leica 2055 microtome for our lab, however it did not come with
an operators manual. Does anyone have one they could e-mail to me? Thank
you
------------------------------
Message: 7
Date: Tue, 29 Dec 2009 20:37:52 -0500
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] Stain to differentiate Hemoglobin from
Hemosiderin?
To: JR R <rosenfeldtek <@t> hotmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <fc31fb4b684f7.4b3a68a0 <@t> uwo.ca>
Content-Type: text/plain; charset=windows-1252
Please explain. The iron in haemoglobin is tightly protein-bound and not
stainable by histochemical methods for the iron in haemosiderin and
ferritin. Red blood cells are, for example, Prussian-blue negative.
John Kiernan
UWO. London, Canada
== == ==
----- Original Message -----
From: JR R <rosenfeldtek <@t> hotmail.com>
Date: Monday, December 28, 2009 19:54
Subject: [Histonet] Stain to differentiate Hemoglobin from Hemosiderin?
To: histonet <@t> lists.utsouthwestern.edu
>
> I've seen Prussian blue as a stain for hemosiderin but that
> would also stain hemoglobin.
>
> Any ideas?
>
> Thanks,
>
> Jerry Ricks
> Research Scientist
> University of Washington
> Department of Pathology
>
> _________________________________________________________________
> Hotmail: Trusted email with Microsofts powerful SPAM protection.
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------------------------------
Message: 8
Date: Wed, 30 Dec 2009 12:17:15 +0200
From: <birnbaumm <@t> asaf.health.gov.il>
Subject: [Histonet] Probe for mouse Y chromosome for FISH
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<054452CCC076BE4DA21E46AE95E32EC3277BE9 <@t> mail2.asaf.health.gov.il>
Content-Type: text/plain; charset="windows-1255"
Dear all
We look for Fluorescence probe of mouse Y chromosome. Which company does
sale it?
Mira Birnbaum
Pathology
Asaf Hrofeh Medical Center
Israel
------------------------------
Message: 9
Date: Wed, 30 Dec 2009 08:17:46 -0500
From: "Demarinis, Carolyn" <cdemarinis <@t> SARATOGACARE.ORG>
Subject: [Histonet] FNA code
To: <HISTONET <@t> PATHOLOGY.SWMED.EDU>
Message-ID:
<F15F698E03068A4CA11D19D3E0ED2815066E29A6 <@t> shexch1.saratogacare.org>
Content-Type: text/plain; charset="us-ascii"
Which CPT code are labs using for fine needle aspirations that are
processed using thinprep technique -
FNA interpretation and report-88173 or
thinprep non-gyn 88112? Thank you.
This e-mail communication and any attachments may contain
confidential and privileged information for the use of the
designated recipients named above.
If you are not the intended recipient, you are hereby notified
that you have received this communication in error and that
any review, disclosure, dissemination, distribution or copying
of it or its contents is prohibited. If you have received this
communication in error, please notify Saratoga Hospital
immediately by e-mail at privacy <@t> saratogacare.org and
destroy all copies of this communication and any attachments.
------------------------------
Message: 10
Date: Wed, 30 Dec 2009 09:40:09 -0500
From: Eric Sulkosky <esulkosky <@t> gmail.com>
Subject: [Histonet] Derm Lab
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6c3840890912300640p70132627w9befa4fcca15117f <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
RAJ,
What tips are you looking for? Are you starting from scratch with an empty
room or has the room already been equipped with ventilation, plumbing and
electrical?
Eric
------------------------------
Message: 11
Date: Wed, 30 Dec 2009 09:42:03 -0500
From: DKBoyd <@t> chs.net
Subject: Re: [Histonet] FNA code
To: "Demarinis, Carolyn" <cdemarinis <@t> SARATOGACARE.ORG>
Cc: histonet-bounces <@t> lists.utsouthwestern.edu,
HISTONET <@t> PATHOLOGY.SWMED.EDU
Message-ID:
<OF9C7D5EE1.697484AB-ON8525769C.0050BC7A-8525769C.0050BA4F <@t> chs.net>
Content-Type: text/plain; charset="US-ASCII"
88173
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F:
804-765-5582 l dkboyd <@t> chs.net
"Demarinis, Carolyn" <cdemarinis <@t> SARATOGACARE.ORG>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
12/30/2009 08:18 AM
To
<HISTONET <@t> PATHOLOGY.SWMED.EDU>
cc
Subject
[Histonet] FNA code
Which CPT code are labs using for fine needle aspirations that are
processed using thinprep technique -
FNA interpretation and report-88173 or
thinprep non-gyn 88112? Thank you.
This e-mail communication and any attachments may contain
confidential and privileged information for the use of the
designated recipients named above.
If you are not the intended recipient, you are hereby notified
that you have received this communication in error and that
any review, disclosure, dissemination, distribution or copying
of it or its contents is prohibited. If you have received this
communication in error, please notify Saratoga Hospital
immediately by e-mail at privacy <@t> saratogacare.org and
destroy all copies of this communication and any attachments.
_______________________________________________
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Message: 12
Date: Wed, 30 Dec 2009 16:37:58 +0100
From: Xipamanine Mkuze <xipamanine <@t> gmail.com>
Subject: Re: [Histonet] Probe for mouse Y chromosome for FISH
To: birnbaumm <@t> asaf.health.gov.il
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<3cfdeb590912300737g155fbd26v1e2a7b0ed98d387d <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Cambio (http://www.cambio.co.uk)
2009/12/30 <birnbaumm <@t> asaf.health.gov.il>
> Dear all
>
> We look for Fluorescence probe of mouse Y chromosome. Which company does
> sale it?
>
> Mira Birnbaum
>
> Pathology
>
> Asaf Hrofeh Medical Center
>
> Israel
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 13
Date: Wed, 30 Dec 2009 11:43:31 -0500
From: "Feher, Stephen" <sfeher <@t> CMC-NH.ORG>
Subject: RE: [Histonet] LEICA 2055 AUTOCUT
To: "Lucy Zong" <lucy.zong <@t> gmail.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<73A7ED895EE0C24D9267ED814911DF1912B74914 <@t> exchange.cmc-nh.org>
Content-Type: text/plain; charset="us-ascii"
I have one for the RM2255 if you think that will help.
Steve
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lucy
Zong
Sent: Tuesday, December 29, 2009 6:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] LEICA 2055 AUTOCUT
I was given a Leica 2055 microtome for our lab, however it did not come
with an operators manual. Does anyone have one they could e-mail to me?
Thank you _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Wed, 30 Dec 2009 09:08:15 -0800 (PST)
From: Jerry Santiago <jsantiago <@t> bellsouth.net>
Subject: [Histonet] Mississippi Histotechnology Homecoming
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <861067.47883.qm <@t> web180409.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=utf-8
Mississippi Histotechnology Homecoming
Calling all Histotechnologists from the State of Mississippi. The awaited
homecoming for the Mississippi Society of Histotechnology is finally here.B
The meeting information will be available in January 2010. To receive the
information, please send an e-mail with name and address to my attention to:
jsantiago <@t> bellsouth.net.
Event: Mississippi Histotechnology Homecoming Event
Dates: March 12 b
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