[Histonet] Stain to differentiate Hemoglobin from Hemosiderin?

John Kiernan jkiernan <@t> uwo.ca
Tue Dec 29 19:37:52 CST 2009

Please explain. The iron in haemoglobin is tightly protein-bound and not stainable by histochemical methods for the iron in haemosiderin and ferritin. Red blood cells are, for example, Prussian-blue negative. 
John Kiernan
UWO.  London,  Canada
== == == 
----- Original Message -----
From: JR R <rosenfeldtek <@t> hotmail.com>
Date: Monday, December 28, 2009 19:54
Subject: [Histonet] Stain to differentiate Hemoglobin from Hemosiderin?
To: histonet <@t> lists.utsouthwestern.edu

> I've seen Prussian blue as a stain for hemosiderin but that 
> would also stain hemoglobin.
> Any ideas?
> Thanks,
> Jerry Ricks
> Research Scientist
> University of Washington
> Department of Pathology
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