[Histonet] Sanderson's rapid bone stain + Acid fuchsin

[sklan <@t> illinois.edu]

Hi All -

I am trying to figure out if the combination of Sanderson's rapid bone stain and acid fuchsin as the counterstain (or methylene blue + basic fuchsin) results in pink/red staining of biologically formed apatite (apatite not associated with collagen produced by osteoblasts)?  An associated question is if the dye-tissue interaction between acid or basic fuchsin and mineralized bone is due to dye-collagen interactions?

If anyone has any experiences or references that may help, please let me know.

Thank you for your help,

Sheeny