[Histonet] basic IHC guide

John Kiernan jkiernan <@t> uwo.ca
Sat Dec 12 22:30:26 CST 2009

Dear Anthony Sandoval,

It's a breath of fresh air to see someone using Histonet to ask about a book rather than ask for a "protocol". 

For simple but thorough explanations and easily followed instructions I strongly recommend
Polak, J.M., and Van Noorden, S. (1997) Royal Microscopical Society Microscopy Handbooks, 37: Introduction to Immunocytochemistry, 2nd ed. Oxford: BIOS Scientific Publications.

There is a 3rd edition (2003) that I don't have. It sells for US$61.24 on Amazon.com:

The 2nd edition, 160-page paperback, cost me 12 pounds (about 20 US$) about 10 years ago. There will be second-hand copies on the market, but I'm not selling mine.

Despite its title, this is a book about immunohistochemistry! (This paragraph is for those of us who don't like "immunocytochemistry" or think the word should be reserved for methods applied to cell cultures, smears of exfoliated cells, blood films, cytocentrifuge preps etc.)

Susan Van Noorden also wrote an excellent chapter about immunohistochemistry in a Biochemical Society publication (2002):
Microscopy and Histology for Molecular Biologists: A User's Guide. London: Portland Press. - ISBN 1855781417 [hard cover]; ISBN 1855781565 [paperback]
Unfortunately, this is a rather expensive book (50 pounds for the paperback or 110 pounds for the hardback). 
The Biochemical Society's market is labs (paperback) and libraries (hardcover), rather than individuals. SVN's chapter epitomizes the content the book she co-authored with Julia Polak. 

John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: Anthony Sandoval <sandoval.1965 <@t> hotmail.com>
Date: Saturday, December 12, 2009 16:00
Subject: [Histonet] basic IHC guide
To: histo net <histonet <@t> lists.utsouthwestern.edu>

> Hello Histonet,
> I am pretty new to IHC and would like to study a basic text 
> book. Can anyone 
> give me a recommendation? Thank you for the support! 
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> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
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