[Histonet] Re: alternates to absolute ethanol

Akemi Allison akemiat3377 <@t> yahoo.com
Fri Dec 11 13:17:23 CST 2009


American Mastertech has a Reagent Grade Ethyl Alcohol which is  
formulated with 95%, 200 proof ethyl alcohol and denatured with 5%  
isopropyl alcohol, NO Methanol!  Cat #ALREACS for 4 Gal.  It costs a  
little more, but well worth it! If you read the fine print on the  
contents of most of your histo and reagent grade alcohols, it is  
mostly denatured with Methanol.

We would use this American Mastertech Reagent  grade for our H&E, IHC  
and Special Stains, stain line.  We also made ALL of our products  
with this grade while I was at Biocare.

Akemi Allison BS, HT (ASCP) HTL
Director
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: akemiat3377 <@t> yahoo.com

On Dec 11, 2009, at 11:39 AM, Robert Richmond wrote:

> The commonly used (in medical histology anyway) alcohol is 90%
> ethanol, 5% methanol, and 5% isopropanol. Since it's denatured, you
> don't have the nuisance and taxes of handling beverage-quality
> ethanol. The common name for this mix is "reagent alcohol". Other
> denaturants such as methylisobutylketone (MIBK) (smells bad) and
> acetone (removes eosin) should be avoided.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
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