[Histonet] time tissue stays in paraffin

[Rene J Buesa]

If this a frequent happening and the tissues stay an extra 30 to 60 minutes, it is not a big deal.
You could to 2 things:
1- set the tissue processor "in delay" mode so it starts 30 to 60 minutes later,and by doing so the tissue will stay from 30-60 minutes extra in NBF, which is good, or
2- program in a way  that the tissue will stay in paraffin 2 and 3 a total of 30-60 mins less, and your tissue will stay the same amount of time in paraffin as if they were not stuck in station 1.
But, again, that is not a big deal.
René J.


--- On Thu, 12/10/09, Dunlap, Charles <DunlapC <@t> umkc.edu> wrote:


From: Dunlap, Charles <DunlapC <@t> umkc.edu>
Subject: [Histonet] time tissue stays in paraffin
To: histonet <@t> lists.utsouthwestern.edu
Date: Thursday, December 10, 2009, 10:56 AM


We have a software glitch in our tissue processor, it  stops in the
first paraffin and will not progress to paraffins 2 and 3 then complete
the cycle.   If we catch it quickly, we can instruct it to continue but
the cycle happens around 5:00 am so we sometimes are not here, tissuet
may spend an extra 30 minutes or even an hour in the first paraffin.
Estimated cost of repairing is a whopping  6 thousand $$.    Does this
have a major effect on quality of slides....sometimes ours are less than
ideal but I am not sure if the problem is in the paraffin cycle or
elsewhere...sectioning, staining, etc.  Any ideas?   Charles Dunlap

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