[Histonet] RE: Help! Losing sections from Superfrost Plus Slides

[sarah tarran]

Hi Angelina,

 

We were having the same problem with frozen mouse aortas. I was cutting at 5ums onto superfrost slides and was losing about half of my sections. I ended up swapping to superfrost plus ultra, plus I bake the slides in the histology oven at 60 degress celcius for 10 minutes and now I nearly never lose a section - the only time I do is when I have done a lazy job of cutting and have collected a section with a fold in it.

Good luck!
 
> 
> Message: 9
> Date: Wed, 09 Dec 2009 11:40:50 -0800
> From: Angelina Fong <fong <@t> zoology.ubc.ca>
> Subject: [Histonet] Help! Losing sections from Superfrost Plus Slides
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <4B1FFD42.2040406 <@t> zoology.ubc.ca>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
> 
> Hi all,
> 
> We have suddenly started losing tissue sections from our Superfrost Plus 
> Slides. 
> 
> Our students have been cutting fixed, frozen, cryosections (20um) and 
> thaw-mounting these onto Superfrost Plus slides. We have suddenly 
> started losing lots of sections from these slides (again!!). The tissue 
> is small - ie cross sections of frog aorta and longitudinal sections of 
> nerves, so any lose of adhesion results in total loss of the tissue. 
> The odd thing is that she is not losing every section on every slide, 
> but half to 3/4 of the sections are falling off within the first rinse. 
> The sections are from the same tissue block on the same slide while some 
> falls and others don't.
> 
> We are at a lose as to what we can do to rescue these sections.
> 
> Does anyone know if there is any way to coat the slides in some solution 
> with the tissue on them to help improve the adhesion without losing the 
> ability to do immunofluorescence?
> 
> Any further advice on cutting / drying protocols are welcomed. 
> 
> This keeps happening and the inconsistency of it has us so frustrated 
> with this that we are thinking of going back to subbing our own slides.
> 
> Thanks for your help!
> 
> Angelina
> 
> 
> -- 
> ~~o~~o~~o~~o~~o~~o~~o~~o~~o~~o
> 
> Angelina Y. Fong, Ph.D.
> Department of Zoology
> Biological Sciences Building
> 6270 University Boulevard
> University of British Columbia
> Vancouver, BC, V6T 1Z4
> Canada 
> 
> Ph: (604) 822-5799
> Fax: (604) 822-2416
> Email: fong <@t> zoology.ubc.ca
> 
> 
> 
> 
> 
> ------------------------------
> 
> Message: 10
> Date: Wed, 9 Dec 2009 15:08:57 -0500
> From: HistoLab <histosearch <@t> gmail.com>
> Subject: [Histonet] Help! Losing sections from Superfrost Plus Slides
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <521c6d260912091208n421bf467g3240121cd26f06a0 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
> 
> Angelina,
> 
> I have seen this post a few times before and sometimes the problem was the
> drying technique or making sure your water bath is clean and free of any
> debris. I also remember hearing about someone pre-treating their slides with
> a Trilogy (EDTA) buffer in a pressure cooker.
> 
> Good Luck!
> 
> *Matthew Semovoski*
> Sales Manager
> Gorilla Scientific Corporation
> 1-866-435-4977
> www.gorillascientific.com
 		 	   		  
_________________________________________________________________
Get more out of Hotmail Check out the latest features today
http://windowslive.ninemsn.com.au/hotmail/article/878466/your-hotmail-is-about-to-get-even-better