[Histonet] TSA

[alonso.martinezcanabal <@t> utoronto.ca]

   Hi,
        We are working here with TSA amplification kits, using them in  
free floating sections or mounted sections. Normally our sections are  
thick, like 40um. We consistently have a lack of an even distribution  
of the staining, normally staying in the first couple of microns if  
the antigen is very abundant (like NeuN) or being more even with very  
sparse cells. Does anyone know what couls be happening here, how could  
we solve this? thank you very much.  By the way, anyone knows another  
way to aplify fluorescence signal that does not involve TSA, like ABC  
kit...
    Thank you very much