[Histonet] staining for lipofucsin

[John Kiernan]

How about Lillie's Nile blue A method? 0.05% dye in 1% sulphuric acid, 20 mins; wash in running water; aqueous mounting medium. See J. Histochem. Cytochem. 4: 377-381 (1956). Anyone can download this paper for free at www.jhc.org.
 
John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: Patsy Ruegg <pruegg <@t> ihctech.net>
Date: Friday, December 4, 2009 13:12
Subject: [Histonet] staining for lipofucsin
To: histonet <@t> lists.utsouthwestern.edu

> Does anyone have recommendations for what special stains to use 
> for ffpe rat
> tissue when we are looking for lipofucsin?  Some of the 
> special stains we
> have investigated include Schmorl's, SBB, Toludine Blue, but the
> investigator is not really satisfied and expects better 
> results.  If anyone
> has experience with trying to stain lipofucsin in tissue please 
> let me hear
> from you.
> 
>  
> 
> Best regards,
> 
>  
> 
> Patsy
> 
>  
> 
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech, LLC
> Fitzsimmons BioScience Park
> 12635 Montview Blvd. Suite 215
> Aurora, CO 80010
> P-720-859-4060
> F-720-859-4110
> wk email pruegg <@t> ihctech.net
> web site www.ihctech.net
> 
>  
> 
> 
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