[Histonet] EM processing

[Va Paula Sicurello]

I have never heard of an EM lab making their own resin for embedding.  We almost all buy the resin kits or the separate components to make either a Epon 812 equivalent or Spurrs.

The amount of time it takes to process is usually tissue dependent.  On average, it takes one day to process (if the tissue is 1mm cubed or less) and 2 days in the oven for the resin to polymerize.  You can speed things up if you microwave process.

If you have any questions, please feel free to contact me.

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF) 
Core for Micro Imaging(C-MI)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397

C-MI for your imaging needs.


--- On Sat, 8/29/09, histopatty <@t> aol.com <histopatty <@t> aol.com> wrote:

> From: histopatty <@t> aol.com <histopatty <@t> aol.com>
> Subject: [Histonet] EM processing
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Saturday, August 29, 2009, 3:36 AM
> Does your lab make their own plastic
> resin to process and embed specimens or do you buy it
> commercially?
> What is the average time it take to process a specimen?
> 
> Thanks in advance to all who respond.
> P.Eneff
> OU Medical Center
> Oklahoma City, OK
> 
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